/OT2-SARS-CoV2

Scripts for automated SARS-CoV2 diagnostic assay on Opentrons liquid handling robot

Primary LanguagePython

OT2-SARS-CoV2

OT-2 API V2 scripts for Opentrons robot using the TaqPath COVID-19 Combo Kit

This workflow enables end-to-end RT-qPCR using the OT2 liquid handling robot by OpenTrons. This includes:

  • Executing magnetic beads-based RNA extraction.
  • Performing qPCR assay preperation protocol in 96-well plate format.

Each folder contains the python script to run the protocol fully in the OT2 desktop application, as well as a Jupyter Notebook that allows the user to test each liquid transfer function individually.

Full setup instructions can be found here

Software Requirements

  1. Python 3

  2. Jupyter Notebook

  3. OT2 APP

  4. Clone this repository to a local computer.

Magnetic Beads-based RNA Extraction

Kit: MagMAX™ Viral/Pathogen II Nucleic Acid Isolation Kit

Sample Volume: 200μL

qPCR Preperation

Kit: TaqPath™ COVID-19 Combo Kit

Sample Volume: 200μL

Authors