Seurat SCtransform
dekan-aleksandr opened this issue · 1 comments
dekan-aleksandr commented
Hello developers of too-many-cells!
Could I use SCtranfrom normalized values as input? Does it contradict the assumptions of your algorithm?
GregorySchwartz commented
The underlying model uses cosine similarity between feature vectors to define similar cells. If that is not appropriate for with SCtransform, then it should not be used, but otherwise it should be fine. Just make sure to disable TooManyCells normalizations / filtering if you are already providing that.