BeerDeCoded is a project carried out by members of Hackuarium, a Swiss not-for-profit association that supports unconventional research ideas and promote the public understanding of science by making laboratory tools more accessible. The goal of BeerDeCoded is to extend scientific knowledge about beer, while discussing issues related to personal genomics, food technology, and their role in society with the general public. Two years ago, a crowdfunding campaign provided funding for the first stage of the project. Reaching out through this channel also allowed to collect 120 beer samples from 20 countries. We have now obtained the metagenomic profiles for 39 of these beers using a targeted approach (ITS). We have demonstrated that it is possible to extract DNA directly from bottled beer using low cost methodologies available to citizen scientists. DNA sequenced from these samples identified a variety of wild yeast species in commercial beers. For example, some brews contain traces of more than 30 different fungal species. Brewing is a complex process and it is unclear if the beer microbiome can directly affect the final beer taste or texture and how it could be controlled to create beers with a specific character.
Altogether, we demonstrated that coupling simple laboratory procedures with DNA sequencing and metagenomic analysis can allow the detection of the microbial content in commercial beer and can easily trigger a tripartite conversation between the general public, the scientists and professional brewers.
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Preliminary analysis of the FASTQ files for 39 Beers The scripts that were used on the cluster: mapping_Beer_to_ITS.sh and BEER_DeCoded_ITS_count.sh
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R statistical analysis of the preprocessed results: Beer_seq.R
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Preprocessed results: BeerDecoded_seq_res_clean.csv or xlsx
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Beer metadata
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Plots
the raw data of our ITS sequencing of the 39 beers will be accessible on the SRA database bioproject ID PRJNA388541