PeterDeWeirdt/cas12a_manuscript

Notebooks for the manuscript Optimization of AsCas12a for combinatorial genetic screens in human cells.

Notebooks for the manuscript Optimization of AsCas12a for combinatorial genetic screens in human cells. Notebooks are organized into sections which correspond to those in the manuscript. The machine learning model to increase guide efficacy relies on the package sgrna_modeler. The apoptosis combinatorial library was analyzed with the package intactr.