GMATA stands for Genome-wide Microsatellite Analyzing Toward Application. GMATA is an easiest and fastest bioinformatic tool /software for any Simple Sequence Repeats (SSR) analyses, and SSR marker designing, polymorphism screen, and e-mapping in any DNA sequences. GMATA algorithms have been cited wordwide for more than 180 times in academic publication.
GMATA uses a range of algorithms to identify tandem repeats and analyze their characteristics, such as the length and purity of the repeat sequence. It can be used to analyze both single sequences and whole genomes, and provides a range of output formats and visualization tools for further analysis.
Overall, GMATA is a useful tool for researchers studying the genetics and genomics of various organisms, as it provides a comprehensive analysis of tandem repeat sequences that can help elucidate their functional and evolutionary significance.
Latest version: v2.3.
Developed by Xuewen Wang.
A new updated version which works on most Linux systems, Windows and MacOS.
Genoytping specified tandem repeat (TR) sites from Next Generation Sequencing platforms can be done using command line from Github https://github.com/XuewenWangUGA/TRcaller. TRcaller which is the fastest (300x human genome sequencing data for 2 seconds for 20 CODIS STRs) and most accurate tool by far for TR genotyping for both short and long NGS sequencing reads.
You can use one of the following methods.
Method 1:
Directly download all files into directory callled GMATA in your computer
Method 2:
git clone https://github.com/XuewenWangUGA/GMATA
cd GMATA
For Linux and Mac OS X, you may need to install additional dependency primer3 and e-PCR.zip which is alvailable at Sourceforge.
Alternatively, Unzip the e-PCR.zip, copy the already pre-compliled excutable files for Linux to the GMATA directory. A precompipled primer3 for Linux is already provided and needed to copy the executable files to the GMATA directory. Linux like systems have to install primer3 depdency. As the latest primer3 IO is changed and compatible to 4 only, the latest Primer3 is not working properly at the moment. Please use the tested version of primer3 (primer3-2.2.3.tar.gz) coming with this package and install it. Tool e-PCR is also included now for those who needs to install in Linux. The instructions for install primers and e-PCR are included after decompressing the .gz or .zip file.
For Windows, everthing is in the package so you don't need to install additional dependency.
more detailed installation steps
Java runtime version 60 or above coming with latest java jdk 16
latest perl 5.##, do not use perl 6.##
latest python3: version 3.6 or above; do not use python 2
latest biopython for python3
latest R 4.##
- run at the graphic interface: This is recommended for who do have programming skills. Only mouse click is needed for this method to run GMATA. or
- run all commands by a single step: This can run batchly and integrate into your pipeline. or
- step by step command
java -jar GMATAv2.3.jar
or double click the GMATA#.jar, where # is the version number, e.g. GMATAV2.2.1.jar
Then you will see the following interface
perl gmata.pl -c default_cfg.txt -i ..\data\testseq.fasta
where -c parameter is for config file. More details and demonstration on user manual File: GMATA%manual_V2.01_20151218.pdf
Please followed the steps in manual and demo.
This is a new function added for discovery and mask genome-wide SSRs
perl gmata.pl -c default_cfg2.2.txt -i /path/gseq.fa
Updated the graphic interface and new module for fastq processing. Fullly updated and compatible with all major versions of Linux including Ubuntu. A new module for converting sequence in fastq to fasta file is added into both command line and graphic interface. To use this function, please install python3 (version 3.6 or above) for Windows. For Linux and Mac OS, the Python3 may be installed by manufacter. The new module is added in graphic interface which can be used by just mouse click. For this new module in command line, please see the detailed usage at my Github Sequence Reads Processing
Updated the graphic interface. fixed the issue which may not start at particular version of Linux Ubuntu.
- add a new module for masking SSRs for genome annotation
- add a new function for satelite analysing
- add tools for mining SSRs in 2nd and 3rd genertation reads
- new interface
This is the software GMATA at GitHUB What is software GMATA Genome-wide Microsatellite Analyzing Toward Application (GMATA) is a software for Simple Sequence Repeats (SSR or STR) identification, and SSR marker designing and polymorhism mapping in any DNA sequences, not matter how big your file is. It has the following functions:
- SSR mining; good for all SSRs:microsatellites, satellites, any tadem repeats
- Statistical analysis and plotting;
- SSR loci graphic viewing;
- Marker designing;
- ploymorphism screen;
- Electronic mapping and marker transferability investigation.
- Sequence file format conversion: Fastq to fasta
GMATA is accurate, sensitive and fast. It was designed to process large genomic sequence data sets, especially large whole genome sequences. In theory, genomes of any size can be analyzed by GMATA easily. Software GMATA works on sever, desktop or even laptop, and it can run in graphic interface with just clicks or run in command line or in automated pipeline. It is also cross-platform and supports Unix/Linux, Win and Mac. Results from software GMATA can be directly graphically displayed with genome or gene features in Gbrowser and easily integrated with any genomic database.
GMATA: an integrated software package for genome-scale SSR mining, marker development and viewing X Wang, L Wang Frontiers in Plant Science 7, doi 10.3389/fpls.2016.01350
The software has been cited more than 300 times in published papers. To see which publication cited GMATA and GMATo, click here at Xuewen Wang Google scholar page
Accurate and fastest SSR mining in any large sequences in fasta or fastq format from NGS sequencing platforms Complete statistical analysis and plotting SSR loci and marker graphic displaying in Gbrowser with genome features Specific SSR marker designing, and simulated PCR Electronic mapping, and marker transferability investigation
To make a test run: go to web https://sourceforge.net/projects/gmata/files/?source=navbar to download the test data called "datav21.zip" or download the "testseq.fasta" and then put a separately folder e.g. "data" instead of running in the source script, otherwise all lots of results will write to the same directory. Of course, it will run well if you still want to keep running the analysis on "testseq.fasta"" in the installed software directory.