workshop-spatial

This repository contains materials and lectures for the joint NBIS and BIIF spatial omics course.

Course content

  • Hands-on experience with ST (Visium), ISS, scRNAseq data analysis
  • Fluorescence-based image formats, standards and quality control
  • Image alignment, registration and ISS decoding
  • Nuclei-based and segmentation-free cell identification
  • Data imputation using ISS and single cell datasets
  • Analysis of Spatial Transcriptomics dataset
  • Cell-type deconvolution (ST and single cell)
  • Cell-cell and ligand-receptor interaction analysis
  • Mapping of multiple spatial data to a common reference
  • High-resolution projection of gene expression to H&E images
  • Interactive visualisation of spatial omics data

PRELIMINARY Schedule


See https://uppsala.instructure.com/courses/58516/pages/schedule


ENTRY REQUIREMENTS:

Application form:

  • Basic knowledge in Python
  • Be able to use your own computer with a web camera
  • Desirable: Previous experience with single cell RNA-seq analysis is an advantage.
  • If we receive more applications, participants will be selected based on several criteria. Selection criteria include correct entry requirements, motivation to attend the course as well as gender and geographical balance.

STAFF:

Course leaders:

  • Christophe Avenel
  • Paulo Czarnewski

Lecturers and content creators:

  • Lars Borm
  • Carolina Wählby
  • Giovanni Palla
  • Naveed Ishaque
  • Anna Shaar
  • David Fischer
  • Hanna Spitzer
  • Sergio Sallas
  • Åsa Björklund
  • Sebastian Tiesmeyer
  • Ludvig Bergenstråle
  • Eduard Chelebian
  • Axel Andersson

Contributing Institutions:

  • Helmholtz-Munich, DE
  • Uppsala University
  • Stockholm University
  • BIH Charité, DE
  • NBIS, SE
  • ELIXIR-SE, ELIXIR-DE
  • EMBO