[Question on the sequencing] Details on the carrier sequencing method

[lucventurini]

Dear authors,
I read today with interest your BMC article on the method implemented in this repository. I am, however, unsure on how it would be replicated experimentally. Specifically, am I correct in understanding that you propose that, in case of a low-concentration sample, the experimenter should "load up" the sample with a known sequence, e.g. the lambda phage used in the article?

If that is the case, I am curious regarding the specific role of the carrier in the reaction. Why would the results be better than without adding the carrier at all? I am afraid that I might have missed the section of the manuscript where you make the argument.

Thank you for your help.

Kind regards

Luca Venturini

[CarrCE]

Hi Luca— Your description is accurate. It is easy to lose a small amount of DNA during library preparation, so using a carrier minimizes any such losses (in addition to maintaining reaction stoichiometry). In addition, nanopore sequencing with little DNA leads to rapid pore burnout. So the carrier acts as a pore-maintainer. Chris

On Thu, Dec 27, 2018 at 8:35 AM Luca Venturini ***@***.***> wrote: Dear authors, I read today with interest your BMC article on the method implemented in this repository. I am, however, unsure on how it would be replicated experimentally. Specifically, am I correct in understanding that you propose that, in case of a low-concentration sample, the experimenter should "load up" the sample with a known sequence, e.g. the lambda phage used in the article? If that is the case, I am curious regarding the specific role of the carrier in the reaction. Why would the results be better than without adding the carrier at all? I am afraid that I might have missed the section of the manuscript where you make the argument. Thank you for your help. Kind regards Luca Venturini — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <#7>, or mute the thread <https://github.com/notifications/unsubscribe-auth/AFCXs45wWrkYsV9SUqNZ2f0iXZl02VCgks5u9PbOgaJpZM4Zi9_7> .

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