/ATACseq

Scripts and Functions related to ATACseq data analysis

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ATACseq

Scripts and Functions related to ATACseq data analysis

General Outline

FastQC Trimmomatic Bowtie2 (-X 2000 parameter)

Filter Alignments

Remove alignments that:

  • map to the MT chromosome
  • are duplicate as found by Picard Tools markduplicates
  • have a mapq score < 30
  • are non properly paired as defined by samtools 0x02 flag

Call peaks with MACS2 Differential analysis with DiffBind