basilkhuder/Seurat-to-RNA-Velocity

errors when running kb count

Sunyp-IM opened this issue · 0 comments

Dear All,

I am trying to follow the tutor. When I run
kb count -i transcriptome.idx -g t2g.txt -x 10xv2 --workflow lamanno --loom -c1 cdna_t2c.txt -c2 intron_t2c.txt read_1.fastq.gz read_2.fastq.gz

I get the errors:

Error: kallisto index file not found transcriptome.idx
Error: file not found read_1.fastq.gz
Error: file not found read_2.fastq.gz
[2023-05-13 03:04:36,697]   ERROR [main] An exception occurred

Could you tell me how to get these idx, read_1.fastq.gz, and read_2.fastq.gz files?

Thank you in Advance.