ben-laufer/CpG_Me

Troobleshooting with bioconda install

El-Castor opened this issue · 3 comments

Hi,

I have try to use your WGBS pipeline but when I launch the conda environment install following this command:

conda install -c bioconda trim-galore bismark bowtie2 samtools fastq-screen picard multiqc

I have many error :

conda install -c bioconda trim-galore bismark bowtie2 samtools fastq-screen picard multiqc
Collecting package metadata (current_repodata.json): done
Solving environment: done


==> WARNING: A newer version of conda exists. <==
  current version: 4.7.12
  latest version: 4.8.3

Please update conda by running

    $ conda update -n base conda



## Package Plan ##

  environment location: /opt/share/FLOCAD/userspace/cpichot/miniconda3/envs/CpG_me

  added / updated specs:
    - bismark
    - bowtie2
    - fastq-screen
    - multiqc
    - picard
    - samtools
    - trim-galore


The following packages will be downloaded:

    package                    |            build
    ---------------------------|-----------------
    bowtie-1.2.3               |   py36hdb11119_1         989 KB  bioconda
    bowtie2-2.4.1              |   py36h7f0b59b_2        12.7 MB  bioconda
    brotlipy-0.7.0             |py36h8c4c3a4_1000         346 KB  conda-forge
    bz2file-0.98               |             py_0           9 KB  conda-forge
    cffi-1.14.0                |   py36hd463f26_0         221 KB  conda-forge
    chardet-3.0.4              |py36h9f0ad1d_1006         188 KB  conda-forge
    cryptography-2.9.2         |   py36h45558ae_0         613 KB  conda-forge
    cutadapt-1.18              |   py36h14c3975_1         206 KB  bioconda
    fastq-screen-0.14.0        |          pl526_0         1.8 MB  bioconda
    hisat2-2.2.0               |   py36h831f99a_3        13.3 MB  bioconda
    idna-2.9                   |             py_1          52 KB  conda-forge
    krb5-1.17.1                |       hfafb76e_1         1.5 MB  conda-forge
    libgd-2.2.5                |    h307a58e_1007         302 KB  conda-forge
    perl-gd-2.71               |  pl526he860b03_0         114 KB  bioconda
    perl-gdgraph-1.54          |          pl526_0          61 KB  bioconda
    perl-gdtextutil-0.86       |  pl526h14c3975_5          21 KB  bioconda
    pigz-2.3.4                 |       hed695b0_1          76 KB  conda-forge
    pycparser-2.20             |             py_0          89 KB  conda-forge
    pyopenssl-19.1.0           |             py_1          47 KB  conda-forge
    pysocks-1.7.1              |   py36h9f0ad1d_1          27 KB  conda-forge
    python-3.6.10              |h8356626_1011_cpython        34.1 MB  conda-forge
    requests-2.24.0            |     pyh9f0ad1d_0          47 KB  conda-forge
    trim-galore-0.6.5          |                0          40 KB  bioconda
    urllib3-1.25.9             |             py_0          92 KB  conda-forge
    xopen-0.7.3                |             py_0          11 KB  bioconda
    ------------------------------------------------------------
                                           Total:        66.8 MB

The following NEW packages will be INSTALLED:

  _libgcc_mutex      conda-forge/linux-64::_libgcc_mutex-0.1-conda_forge
  _openmp_mutex      conda-forge/linux-64::_openmp_mutex-4.5-1_llvm
  _r-mutex           conda-forge/noarch::_r-mutex-1.0.1-anacondar_1
  alsa-lib           conda-forge/linux-64::alsa-lib-1.1.5-h516909a_1002
  binutils_impl_lin~ conda-forge/linux-64::binutils_impl_linux-64-2.34-h53a641e_5
  binutils_linux-64  conda-forge/linux-64::binutils_linux-64-2.34-hc952b39_20
  bismark            bioconda/noarch::bismark-0.22.3-0
  bowtie             bioconda/linux-64::bowtie-1.2.3-py36hdb11119_1
  bowtie2            bioconda/linux-64::bowtie2-2.4.1-py36h7f0b59b_2
  brotlipy           conda-forge/linux-64::brotlipy-0.7.0-py36h8c4c3a4_1000
  bwidget            conda-forge/linux-64::bwidget-1.9.14-0
  bz2file            conda-forge/noarch::bz2file-0.98-py_0
  bzip2              conda-forge/linux-64::bzip2-1.0.8-h516909a_2
  ca-certificates    conda-forge/linux-64::ca-certificates-2020.6.20-hecda079_0
  cairo              conda-forge/linux-64::cairo-1.16.0-hcf35c78_1003
  certifi            conda-forge/linux-64::certifi-2020.6.20-py36h9f0ad1d_0
  cffi               conda-forge/linux-64::cffi-1.14.0-py36hd463f26_0
  chardet            conda-forge/linux-64::chardet-3.0.4-py36h9f0ad1d_1006
  click              conda-forge/noarch::click-7.1.2-pyh9f0ad1d_0
  coloredlogs        conda-forge/linux-64::coloredlogs-14.0-py36h9f0ad1d_1
  colormath          conda-forge/noarch::colormath-3.0.0-py_2
  cryptography       conda-forge/linux-64::cryptography-2.9.2-py36h45558ae_0
  curl               conda-forge/linux-64::curl-7.69.1-h33f0ec9_0
  cutadapt           bioconda/linux-64::cutadapt-1.18-py36h14c3975_1
  cycler             conda-forge/noarch::cycler-0.10.0-py_2
  decorator          conda-forge/noarch::decorator-4.4.2-py_0
  expat              conda-forge/linux-64::expat-2.2.9-he1b5a44_2
  fastq-screen       bioconda/noarch::fastq-screen-0.14.0-pl526_0
  fastqc             bioconda/noarch::fastqc-0.11.9-0
  font-ttf-dejavu-s~ conda-forge/noarch::font-ttf-dejavu-sans-mono-2.37-hab24e00_0
  fontconfig         conda-forge/linux-64::fontconfig-2.13.1-h86ecdb6_1001
  freetype           conda-forge/linux-64::freetype-2.10.2-he06d7ca_0
  fribidi            conda-forge/linux-64::fribidi-1.0.9-h516909a_0
  future             conda-forge/linux-64::future-0.18.2-py36h9f0ad1d_1
  gcc_impl_linux-64  conda-forge/linux-64::gcc_impl_linux-64-7.5.0-hd420e75_6
  gcc_linux-64       conda-forge/linux-64::gcc_linux-64-7.5.0-h09487f9_20
  gettext            conda-forge/linux-64::gettext-0.19.8.1-hc5be6a0_1002
  gfortran_impl_lin~ conda-forge/linux-64::gfortran_impl_linux-64-7.5.0-hdf63c60_6
  gfortran_linux-64  conda-forge/linux-64::gfortran_linux-64-7.5.0-h09487f9_20
  giflib             conda-forge/linux-64::giflib-5.2.1-h516909a_2
  glib               conda-forge/linux-64::glib-2.65.0-h6f030ca_0
  graphite2          conda-forge/linux-64::graphite2-1.3.13-he1b5a44_1001
  gsl                conda-forge/linux-64::gsl-2.6-h294904e_0
  gxx_impl_linux-64  conda-forge/linux-64::gxx_impl_linux-64-7.5.0-hdf63c60_6
  gxx_linux-64       conda-forge/linux-64::gxx_linux-64-7.5.0-h09487f9_20
  harfbuzz           conda-forge/linux-64::harfbuzz-2.4.0-h9f30f68_3
  hisat2             bioconda/linux-64::hisat2-2.2.0-py36h831f99a_3
  htslib             bioconda/linux-64::htslib-1.9-h4da6232_3
  humanfriendly      conda-forge/linux-64::humanfriendly-8.2-py36h9f0ad1d_0
  icu                conda-forge/linux-64::icu-64.2-he1b5a44_1
  idna               conda-forge/noarch::idna-2.9-py_1
  importlib-metadata conda-forge/linux-64::importlib-metadata-1.6.1-py36h9f0ad1d_0
  jinja2             conda-forge/noarch::jinja2-2.11.2-pyh9f0ad1d_0
  jpeg               conda-forge/linux-64::jpeg-9d-h516909a_0
  kiwisolver         conda-forge/linux-64::kiwisolver-1.2.0-py36hdb11119_0
  krb5               conda-forge/linux-64::krb5-1.17.1-hfafb76e_1
  lcms2              conda-forge/linux-64::lcms2-2.11-hbd6801e_0
  ld_impl_linux-64   conda-forge/linux-64::ld_impl_linux-64-2.34-h53a641e_5
  libblas            conda-forge/linux-64::libblas-3.8.0-16_openblas
  libcblas           conda-forge/linux-64::libcblas-3.8.0-16_openblas
  libcurl            conda-forge/linux-64::libcurl-7.69.1-hf7181ac_0
  libdeflate         bioconda/linux-64::libdeflate-1.2-h516909a_1
  libedit            conda-forge/linux-64::libedit-3.1.20191231-h46ee950_0
  libffi             conda-forge/linux-64::libffi-3.2.1-he1b5a44_1007
  libgcc-ng          conda-forge/linux-64::libgcc-ng-9.2.0-h24d8f2e_2
  libgd              conda-forge/linux-64::libgd-2.2.5-h307a58e_1007
  libgfortran-ng     conda-forge/linux-64::libgfortran-ng-7.5.0-hdf63c60_6
  libgomp            conda-forge/linux-64::libgomp-9.2.0-h24d8f2e_2
  libiconv           conda-forge/linux-64::libiconv-1.15-h516909a_1006
  liblapack          conda-forge/linux-64::liblapack-3.8.0-16_openblas
  libopenblas        conda-forge/linux-64::libopenblas-0.3.9-h5ec1e0e_0
  libpng             conda-forge/linux-64::libpng-1.6.37-hed695b0_1
  libssh2            conda-forge/linux-64::libssh2-1.9.0-hab1572f_2
  libstdcxx-ng       conda-forge/linux-64::libstdcxx-ng-9.2.0-hdf63c60_2
  libtiff            conda-forge/linux-64::libtiff-4.1.0-hc3755c2_3
  libuuid            conda-forge/linux-64::libuuid-2.32.1-h14c3975_1000
  libwebp            conda-forge/linux-64::libwebp-1.0.2-h56121f0_5
  libxcb             conda-forge/linux-64::libxcb-1.13-h14c3975_1002
  libxml2            conda-forge/linux-64::libxml2-2.9.10-hee79883_0
  llvm-openmp        conda-forge/linux-64::llvm-openmp-10.0.0-hc9558a2_0
  lz4-c              conda-forge/linux-64::lz4-c-1.9.2-he1b5a44_1
  lzstring           conda-forge/noarch::lzstring-1.0.4-py_1001
  make               conda-forge/linux-64::make-4.3-h516909a_0
  markdown           conda-forge/noarch::markdown-3.2.2-py_0
  markupsafe         conda-forge/linux-64::markupsafe-1.1.1-py36h8c4c3a4_1
  matplotlib-base    conda-forge/linux-64::matplotlib-base-3.2.2-py36hb8e4980_0
  multiqc            bioconda/noarch::multiqc-1.9-pyh9f0ad1d_0
  ncurses            conda-forge/linux-64::ncurses-6.1-hf484d3e_1002
  networkx           conda-forge/noarch::networkx-2.4-py_1
  numpy              conda-forge/linux-64::numpy-1.18.5-py36h7314795_0
  openjdk            conda-forge/linux-64::openjdk-11.0.1-h600c080_1018
  openssl            conda-forge/linux-64::openssl-1.1.1g-h516909a_0
  pango              conda-forge/linux-64::pango-1.42.4-h7062337_4
  pcre               conda-forge/linux-64::pcre-8.44-he1b5a44_0
  pcre2              conda-forge/linux-64::pcre2-10.35-h2f06484_0
  perl               conda-forge/linux-64::perl-5.26.2-h516909a_1006
  perl-gd            bioconda/linux-64::perl-gd-2.71-pl526he860b03_0
  perl-gdgraph       bioconda/linux-64::perl-gdgraph-1.54-pl526_0
  perl-gdtextutil    bioconda/linux-64::perl-gdtextutil-0.86-pl526h14c3975_5
  picard             bioconda/noarch::picard-2.23.0-0
  pigz               conda-forge/linux-64::pigz-2.3.4-hed695b0_1
  pip                conda-forge/noarch::pip-20.1.1-py_1
  pixman             conda-forge/linux-64::pixman-0.38.0-h516909a_1003
  pthread-stubs      conda-forge/linux-64::pthread-stubs-0.4-h14c3975_1001
  pycparser          conda-forge/noarch::pycparser-2.20-py_0
  pyopenssl          conda-forge/noarch::pyopenssl-19.1.0-py_1
  pyparsing          conda-forge/noarch::pyparsing-2.4.7-pyh9f0ad1d_0
  pysocks            conda-forge/linux-64::pysocks-1.7.1-py36h9f0ad1d_1
  python             conda-forge/linux-64::python-3.6.10-h8356626_1011_cpython
  python-dateutil    conda-forge/noarch::python-dateutil-2.8.1-py_0
  python_abi         conda-forge/linux-64::python_abi-3.6-1_cp36m
  pyyaml             conda-forge/linux-64::pyyaml-5.3.1-py36h8c4c3a4_0
  r-base             conda-forge/linux-64::r-base-4.0.1-h95c6c4b_0
  readline           conda-forge/linux-64::readline-8.0-hf8c457e_0
  requests           conda-forge/noarch::requests-2.24.0-pyh9f0ad1d_0
  samtools           bioconda/linux-64::samtools-1.9-h10a08f8_12
  sed                conda-forge/linux-64::sed-4.7-h1bed415_1000
  setuptools         conda-forge/linux-64::setuptools-47.3.1-py36h9f0ad1d_0
  simplejson         bioconda/linux-64::simplejson-3.8.1-py36_0
  six                conda-forge/noarch::six-1.15.0-pyh9f0ad1d_0
  spectra            conda-forge/noarch::spectra-0.0.11-py_1
  sqlite             conda-forge/linux-64::sqlite-3.30.1-hcee41ef_0
  tbb                conda-forge/linux-64::tbb-2020.1-hc9558a2_0
  tk                 conda-forge/linux-64::tk-8.6.10-hed695b0_0
  tktable            conda-forge/linux-64::tktable-2.10-h555a92e_3
  tornado            conda-forge/linux-64::tornado-6.0.4-py36h8c4c3a4_1
  trim-galore        bioconda/noarch::trim-galore-0.6.5-0
  urllib3            conda-forge/noarch::urllib3-1.25.9-py_0
  wheel              conda-forge/noarch::wheel-0.34.2-py_1
  xopen              bioconda/noarch::xopen-0.7.3-py_0
  xorg-fixesproto    conda-forge/linux-64::xorg-fixesproto-5.0-h14c3975_1002
  xorg-inputproto    conda-forge/linux-64::xorg-inputproto-2.3.2-h14c3975_1002
  xorg-kbproto       conda-forge/linux-64::xorg-kbproto-1.0.7-h14c3975_1002
  xorg-libice        conda-forge/linux-64::xorg-libice-1.0.10-h516909a_0
  xorg-libsm         conda-forge/linux-64::xorg-libsm-1.2.3-h84519dc_1000
  xorg-libx11        conda-forge/linux-64::xorg-libx11-1.6.9-h516909a_0
  xorg-libxau        conda-forge/linux-64::xorg-libxau-1.0.9-h14c3975_0
  xorg-libxdmcp      conda-forge/linux-64::xorg-libxdmcp-1.1.3-h516909a_0
  xorg-libxext       conda-forge/linux-64::xorg-libxext-1.3.4-h516909a_0
  xorg-libxfixes     conda-forge/linux-64::xorg-libxfixes-5.0.3-h516909a_1004
  xorg-libxi         conda-forge/linux-64::xorg-libxi-1.7.10-h516909a_0
  xorg-libxrender    conda-forge/linux-64::xorg-libxrender-0.9.10-h516909a_1002
  xorg-libxtst       conda-forge/linux-64::xorg-libxtst-1.2.3-h516909a_1002
  xorg-recordproto   conda-forge/linux-64::xorg-recordproto-1.14.2-h516909a_1002
  xorg-renderproto   conda-forge/linux-64::xorg-renderproto-0.11.1-h14c3975_1002
  xorg-xextproto     conda-forge/linux-64::xorg-xextproto-7.3.0-h14c3975_1002
  xorg-xproto        conda-forge/linux-64::xorg-xproto-7.0.31-h14c3975_1007
  xz                 conda-forge/linux-64::xz-5.2.5-h516909a_0
  yaml               conda-forge/linux-64::yaml-0.2.5-h516909a_0
  zipp               conda-forge/noarch::zipp-3.1.0-py_0
  zlib               conda-forge/linux-64::zlib-1.2.11-h516909a_1006
  zstd               conda-forge/linux-64::zstd-1.4.4-h6597ccf_3


Proceed ([y]/n)? y


Downloading and Extracting Packages
cryptography-2.9.2   | 613 KB    | ############################################################################# | 100% 
perl-gd-2.71         | 114 KB    | ############################################################################# | 100% 
pyopenssl-19.1.0     | 47 KB     | ############################################################################# | 100% 
chardet-3.0.4        | 188 KB    | ############################################################################# | 100% 
pysocks-1.7.1        | 27 KB     | ############################################################################# | 100% 
bowtie-1.2.3         | 989 KB    | ############################################################################# | 100% 
pigz-2.3.4           | 76 KB     | ############################################################################# | 100% 
bz2file-0.98         | 9 KB      | ############################################################################# | 100% 
xopen-0.7.3          | 11 KB     | ############################################################################# | 100% 
hisat2-2.2.0         | 13.3 MB   | ############################################################################# | 100% 
fastq-screen-0.14.0  | 1.8 MB    | ############################################################################# | 100% 
requests-2.24.0      | 47 KB     | ############################################################################# | 100% 
trim-galore-0.6.5    | 40 KB     | ############################################################################# | 100% 
cutadapt-1.18        | 206 KB    | ############################################################################# | 100% 
idna-2.9             | 52 KB     | ############################################################################# | 100% 
pycparser-2.20       | 89 KB     | ############################################################################# | 100% 
bowtie2-2.4.1        | 12.7 MB   | ############################################################################# | 100% 
perl-gdtextutil-0.86 | 21 KB     | ############################################################################# | 100% 
krb5-1.17.1          | 1.5 MB    | ############################################################################# | 100% 
perl-gdgraph-1.54    | 61 KB     | ############################################################################# | 100% 
python-3.6.10        | 34.1 MB   | ############################################################################# | 100% 
cffi-1.14.0          | 221 KB    | ############################################################################# | 100% 
urllib3-1.25.9       | 92 KB     | ############################################################################# | 100% 
libgd-2.2.5          | 302 KB    | ############################################################################# | 100% 
brotlipy-0.7.0       | 346 KB    | ############################################################################# | 100% 
Preparing transaction: done
Verifying transaction: \ 
SafetyError: The package for r-base located at /opt/share/FLOCAD/userspace/cpichot/miniconda3/pkgs/r-base-4.0.1-h95c6c4b_0
appears to be corrupted. The path 'lib/R/doc/html/packages.html'
has an incorrect size.
  reported size: 2946 bytes
  actual size: 17754 bytes

ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/convert_quals.pl'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/gen_2b_occ_lookup.pl'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/gen_occ_lookup.pl'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/gen_solqual_lookup.pl'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/infer_fraglen.pl'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_a_thaliana_tair.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_b_taurus_UMD3.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_c_elegans.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_canFam2.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_d_melanogaster.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_e_coli.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_h_sapiens_ncbi36.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_h_sapiens_ncbi37.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_hg18.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_hg19.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_m_musculus_ncbi37.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_mm9.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_rn4.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/make_s_cerevisiae.sh'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/test/DNA.pm'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/test/big_data/reads/human_reads.fa'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/test/big_data/reads/mouse_reads.fa'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/test/btdata.py'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/test/dataface.py'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/test/large_idx.py'


ClobberError: This transaction has incompatible packages due to a shared path.
  packages: bioconda::bowtie-1.2.3-py36hdb11119_1, bioconda::bowtie2-2.4.1-py36h7f0b59b_2
  path: 'bin/scripts/test/simple_tests.pl'



done
Executing transaction: done

Are the bioconda package available ?Do you have any suggestion please ?

Thanks in advance

Hi @El-Castor, thanks for getting in touch about this. I think it's worth updating your conda to the newer version, but it looks to me like the error is from Bowtie1 and Bowtie2 clashing, since I believe Bowtie1 is included in Bismark by default. Can you try removing Bowtie2 from the command then just installing it on its own through conda after? Please let me know how that goes.

Hi @ben-laufer,

Whithout bowtie2 it work well, is it already implemented in bismark.

Thank for your fast response and your help.
regards

It does look like Bowtie2 is now a dependency for Bismark in conda, so you should be good to go. Thanks for pointing this out, I'll update the documentation.