bioinfo-biols/CIRIquant

Cannot split into 12 (16) pieces with size of 6246233965 and named them as /oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/APO-noD-R_unmapped.sam. Fatal error. Aborted.

Closed this issue · 2 comments

Hi
I encounter this error when running
CIRIquant -t 4 -1 output/fastqs/APO-1-R_1.Tr.fq.gz -2 output/fastqs/APO-1-R_2.Tr.fq.gz --config ciriconfig_full.yaml --library-type 2 -o output/ -p APO-1-R -t 12

log:

[Mon May 30 18:19:05 2022] Loading reference
[Mon May 30 18:19:39 2022] Requesting system to split SAM into 12 pieces
[Mon May 30 18:19:05 2022] CIRI begins running
[Mon May 30 18:19:05 2022] Loading reference
[Mon May 30 18:19:39 2022] Requesting system to split SAM into 12 pieces
Divided SAM sizes:
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samaa	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samab	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samac	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samad	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samae	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samaf	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samag	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samah	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samai	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samaj	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samak	     6136420186
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samal	     6136420184
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samam	     4602315006
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.saman	     4602315423
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samao	     4602315554
/oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.samap	     4602314573
Cannot split /oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.sam into 12 (16) pieces with size of 6136420186 and named them as /oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.sam.
Fatal error. Aborted.
    please help, thanks. I am pretty suer I have write access to the output dir

Hi @algaebrown , the /oasis/tscc/scratch/hsher/circSTAMP_pipe/output/circ/HuR-1-R_unmapped.sama[m-p] seems to be the temporary files from your last run. Could you delete the output directory and run CIRIquant again?.

Got it. Thanks!!