choderalab/wetlab-protocols

Issues

• Denovix- Not saving the history on Data App

  #49 opened 5 years ago by Binikarki
  0

• Update Quantos S.O.P.

  #41 opened 7 years ago by sonyahanson
  4

• What are our standard Deck Layouts for the EVO?

  #32 opened 8 years ago by sonyahanson
  8

• Add automation checklist for running protocols

  #39 opened 8 years ago by sonyahanson
  1

• Deprecate old versions of SOPs in the wiki

  #34 opened 8 years ago by jchodera
  0

• Update Quantos operation SOPs with stuff from wiki

  #35 opened 8 years ago by jchodera
  0

• Primer selection

  #31 opened 8 years ago by steven-albanese
  2

• Protocol Format Standard

  #30 opened 8 years ago by sonyahanson
  3

• Molecular Biology: Plasmid Preparation buffers

  #29 opened 8 years ago by steven-albanese
  0

• Should we add a density meter protocol here?

  #15 opened 9 years ago by sonyahanson
  2

• Another possibility: Try a commercial S12-lysate system first before producing S12-lysate ourselves?

  #11 opened 9 years ago by jchodera
  10

• Add citations within protocol

  #12 opened 9 years ago by sonyahanson
  1

• How is cell washing done?

  #6 opened 9 years ago by jchodera
  1

• Expression of T7 RNA pol

  #10 opened 9 years ago by jchodera
  1

• JOVE article on in vitro TX-TL protocols

  #9 opened 9 years ago by jchodera
  1

• What happens after centrifuging crude lysate?

  #7 opened 9 years ago by jchodera
  0

• Buffers A and B: DTT, TCEP, and 3-ME

  #5 opened 9 years ago by jchodera
  0

• Note quantity of TCEP to be added per liter of cell-free buffers A and B

  #4 opened 9 years ago by jchodera
  0

• Volume of reaction

  #3 opened 9 years ago by pgrinaway
  0

• Length of reaction

  #2 opened 9 years ago by pgrinaway
  0

• Induction of RNA polymerase

  #1 opened 9 years ago by pgrinaway
  0