[Bug]: It's not possible to basecall without a reference.
mattloose opened this issue · 4 comments
mattloose commented
What happened?
I'd really like to be able to run dorado and generate an unaligned bam file of meth calls. For some reason a reference is compulsory for this workflow?
Operating System
ubuntu 20.04
Workflow Execution
EPI2ME Labs desktop application
Workflow Execution - EPI2ME Labs Versions
No response
Workflow Execution - CLI Execution Profile
Singularity
Workflow Version
revision: 2d733e8 [master]
Relevant log output
ERROR: Validation of pipeline parameters failed!
* Missing required parameter: --refSamStudio8 commented
Thank you for your valuable feedback @mattloose
mattloose commented
You are most welcome @SamStudio8
In the end I just used dorado natively - but we like epi2melabs and are tyring to roll these pipelines out to other users around the university.
SamStudio8 commented
@mattloose v0.4.0 has been updated to remove the requirement for a reference, please give it a try!
mattloose commented
Awesome - thank you.