The Shear Flow Deformation Cytometer is a method to measure the visco-elastic properties of living cells in suspension. Cells are suspended in a high-viscosity (0.5-5 Pa s) fluid (typically alginate with concentrations 1-2.5%) and exposed to fluid shear during their passage through large (200 µm diameter) microfluidic channels.
The software uses Python, so you need to have a Python installation, e.g. Anaconda.
To install the software package, download the repository from GitHub, open a command line in the downloaded folder (the one containing "setup.py") and execute
pip install .
To record image data, use the script:
recording.py
To evaluate the resulting data (.tif files) use the script:
evaluate.py
To analyse the evaluated data, this script example can serve as a starting point.
import numpy as np
import pandas as pd
import matplotlib.pyplot as plt
from deformationcytometer.evaluation.helper_functions import load_all_data_new, get2Dhist_k_alpha_err
# load all the data in the given folder
# you can also add a list of folders instead
# filenames can also include * for wildcard matches
data, config = load_all_data_new(r"\\path_to_folder_containing_the_data")
# print all the columns, these are the ones from the results.csv and from the meta data files
print(data.columns)
# you can now group by some of the columns
# for example just by the filename to get an evaluation per file
for filename, d in data.groupby("filename"):
# calculate the k and alpha and their bootstrapped errors according to the 2D mode
k, k_err, alpha, alpha_err = get2Dhist_k_alpha_err(d)
print(filename, k, k_err, alpha, alpha_err)
# or directly apply it on the grouped dataframe to get a new dataframe
aggregated_data = data.groupby("filename").apply(get2Dhist_k_alpha_err)
print(aggregated_data)