gagneurlab/OUTRIDER

PROTRIDER questions

Opened this issue · 1 comments

m-pauper commented

  1. Since a part of OUTRIDER's vignette was designed with RNA-seq data in mind, it seems that not all functions are meant to be used for proteomics data. For instance, there is the filterExpression step, but it seems that is based on fpkm values and thus should be irrelevant with proteomics data? Similarly, I was unable to use the plotCountGeneSampleHeatmap function. Are there alternatives for those for proteomics data?
    Found it in the vignette:
    • plotExpectedVsObservedCounts -> plotExpectedVsObserved,
    • plotCountCorHeatmap -> plotSampleCorHeatmap,
    • plotCountGeneSampleHeatmap -> plotFeatureSampleHeatmap.

  2. This issue (#32) mentions that the order of the samples in the annotations table should be the same as the order of the rows in the inputData. Is that true?

  3. Edit: moved to: #60

  4. Should input protein intensities be raw values or is it ok if they are already log-transformed?

m-pauper commented
  1. • plotCountCorHeatmap -> plotSampleCorHeatmap,

Actually, plotSampleCorHeatmap does not seem to be available. But it seems that with this:

setMethod("plotCountCorHeatmap", signature="Outrider2DataSet", 
        plotSampleCorHeatmap)

One can still use the plotCountCorHeatmap for an Outrider2DataSet and that will use the plotSampleCorHeatmap.

It would be nice if you could confirm that :)