This is a simple program that reads in a file with multiple sequences in fasta format and then finds the primers that are required to join these sequences together using a yeast assembly protocol. Dependency - primer3 (https://github.com/libnano/primer3-py) You can use the get_primer3.sh script to download and install the primer3 module. This will be done inplace where this repo was cloned.
gerikson/primer_tool
This is a simple program that reads in a file with multiple sequences in fasta format and then finds the primers that are required to join these sequences together using a yeast assembly protocol. Dependency - primer3 (https://github.com/libnano/primer3-py)
Python