Duplicated exons issue
Closed this issue · 2 comments
AngryMaciek commented
Dear Authors,
I am using the official annotation from ENSEMBL but the exons are not unique:
grep ENSE00001957285 Homo_sapiens.GRCh38.87.gtf
(This GTF can be downloaded from the official site: ftp://ftp.ensembl.org/pub/release-87/gtf/homo_sapiens/ )
Also, the conversion method which you provided does not work, I assume that this happens because the file is ENSEMBL annotation(?) Could you please let me know how should I run rnaseqc with this particular GTF version?
agraubert commented
Edit: The latest version of the collapsing script can handle ENSEMBL annotations. I have tested it to verify that it works with the specific gtf you mentioned.
Let me know if this resolves your issue
AngryMaciek commented
I have tested the python script together with RNA-SeQC 2.3.5 and it works fine, thank you very much!