/A188Ref1

the process to build the first A188 reference genome

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A188 Ref1

Liu lab at Kansas State University

A188 genome assembly and annotation

  1. Sequencing data
  • Nanopore data Nanopore data were generated using ONT MinION with the FLO-MIN106 flowcells and the kit of SQK-LSK109. Basecalling was performed with Guppy (3.4.4).
  • Illumina data A188 PE125: BGI
    A188 PE250: Novogene

  1. Canu1.9 assembly

  2. Nanopolish and Pilon polishing

  3. Hybrid scaffolding

  4. Genetic mapping for further scaffolding

  5. Contigs concatenation

  6. Maker genome annotation

  7. Othologs

  8. Analyses related to the manuscript