Export count matrix containing isoforms
grimwoo opened this issue · 1 comments
grimwoo commented
After finishing the brie
, I had three files: fractions.tsv, weights.tsv and samples.csv.gz.
And I need to use Seurat for further analysis.
However, I need, at least, the expression matrix. The format could be as following:
AAACATACCATCAG AAACATACCGATAC AAACATACGCATCA
ENSMUSG00000111425.1 1 2 0
ENSMUSG00000024442.5 0 1 0
OR
Cell_1 Cell_2 Cell_3
Gene_1_isoform_1 1 2 0
Gene_1_isoform_2 0 1 0
Gene_2_isoform_1 1 1 0
Does anyone know how to export a file like this or how to combine BRIE and Seurat?
huangyh09 commented
Hi, Sorry on the delay. It is not straightforward to get the isoform-by-cell matrix in BRIE1, but it should be properly supported in BRIE2 (in AnnData layer).
Yuanhua