confused about Psi_95CI

[QuanlongJiang]

Dear author,

1. I think the psi 95CI shuld be a invterval. but only a single value was provied in the result. Is Psi_95CI the upper bounds of 95 CI?
2. you said " if a splicing event in a gene doesn’t have any read, it will return a posterior with Psi’s mean=0.5 and 95% confidence interval around 0.95 (most cases >0.9)" . I think PSI 0.5 means a half of exon was exclued. This is confused. Is that better if the PSI assigned as 1 if there is no reads?
3. why Psi_95CI > 0.3 keep the confident psi?

Thanks a lot, I hope to hear from you.
quanlong Jiang

[huangyh09]

Hi, thanks for the questions.

1. You are right, 95 CI refers to the lower and upper bound of 95% interval. Here, we compact it to a single value to denote the range (upper - lower bounds), as we aim to use for filtering.
2. that's the impact of how we choose the prior. In BRIE2, it is recommended to use the average of the cell population as the mean of the prior (a logit-normal distribution). Nonetheless, one should keep an eye on the 95 CI to filter out those less confident quantifications.
3. It should be Psi_95CI < 0.3 as confident psi. One can choose a more stringent cutoff, if they are more conservative or have very high coverage.

Yuanhua