This repository contains some scripts that are helpful for preparing Illumina libraries.
pip install git+https://github.com/kerrycobb/lib-prep-tools
Calculate how much starting material and how much water/buffer are needed to achievve a desired nanomolar concentration and volume of DNA for sequencing.
Run samplePrep --help for guide to usage.
Illumina platforms typically require samples to be at 5 nanoMolar concentrations.
Calculate how much starting material and how much water/buffer are needed to achieve a desired concentration and volume of DNA.
Run dilutionCalc --help for guide to usage.
Calculate the concentration of DNA needed to achieve a desired molarity.
Run molarityCalc --help for guide to usage.