/ahr-tead-motif-analysis

Analysis for "Cell-intrinsic Aryl Hydrocarbon Receptor signalling is required for the resolution of injury-induced colonic stem cells"

Primary LanguageJupyter Notebook

Ahr TEAD motif analysis

Analysis repository regarding review comments for:

"Cell-intrinsic Aryl Hydrocarbon Receptor signalling is required for the resolution of injury-induced colonic stem cells"

Prerequisites

  • Linux/Unix platform (macOS supported)
  • Conda installed

Installation

Clone this repository, navigate to the folder using the command line and install the conda environment using

conda env create -f environment.yml

Generating base data

To generate the bed files which are consumed by the downstream analysis, run generate_beds.sh

Performing downstream analysis

To view the downstream analysis just click on analysis.ipynb in the browser on github.

To re-run the analysis, start jupyter by typing jupyter lab from the root repository folder, then open either analysis or ucsc_export and re-run the steps.

UCSC Export

This script will format and normalise the data for display in UCSC browser. All bed files can be directory uploaded from _ucsc_export to the browser as custom tracks.

Analysis

This script contains all the steps to generate the figures and stats used in answering the relevant reviewer questions.