Bug: BEDPE Format input for hmmratac
bcantarel opened this issue · 3 comments
Thanks so much for adding the option to allow bedpe for hmmratac as a way to support CRAM -- unfortunately, when I am trying this command on a BED file, I am getting the following error
Describe the bug
INFO @ 26 Sep 2023 00:19:46: [95 MB]
Command line: hmmratac -i the_BEDPE_file.bed -f BEDPE -n temp
Random seed selected as: 10151
INFO @ 26 Sep 2023 00:19:46: [95 MB] #1 Read fragments from BAM file...
Traceback (most recent call last):
File "/opt/conda/bin/macs3", line 1026, in
main()
File "/opt/conda/bin/macs3", line 101, in main
run( args )
File "/opt/conda/lib/python3.10/site-packages/MACS3/Commands/hmmratac_cmd.py", line 81, in run
bam = BAMPEParser(options.bam_file[0], buffer_size=options.buffer_size)
To Reproduce
samtools view -@ 12 -1 --reference genome.fa -o file.bam file.cram
macs3 randsample -i file.bam -f BAMPE -p 100 -o the_BEDPE_file.bed
macs3 hmmratac -f BEDPE -i the_BEDPE_file.bed --outdir macs3out -n out --save-digested
System (please complete the following information):
- OS: Linux
- Python 3.10.12
- Numpy version 1.23.5
- macs3 3.0.0b3 (pulled from main last week)
It is strange since in the main branch the line 81 of hmmratac_cmd.py is different with what you saw. You saw bam = BAMPEParser(options.bam_file[0], buffer_size=options.buffer_size)
, but now it's
MACS/MACS3/Commands/hmmratac_cmd.py
Lines 78 to 85 in 429f8f8
Since this change hasn't been included in b3, could you remove MACS3 such as pip uninstall macs3
then pull the main branch (git clone git@github.com:macs3-project/MACS.git) and install macs3 again? Sorry that we are working on finishing documentation these days and haven't included this BEDPE feature for hmmratac in a new release.
Thanks! This worked for me!!
@bcantarel FYI, in the release 3.0.0, we have included the support of BEDPE format in hmmratac
command.