mckennalab/FlashFry

Support for Cpf1 and CasΦ

hcph opened this issue · 1 comments

hcph commented

Amazing tool, I really like it since it is fast enough especially when the genome is very large. I am super interested in using this tool to figure out the off targets for Cpf1 and the newly reported CasΦ (PAM=‘TBN’)because they have way smaller size, which brings great potential in future gene therapy. Is it possible to add the off-target scan function for Cpf1 and CasΦ? Waiting for your reply and thank you in advance!

There seems to be a lot of demand for alternative CRISPR enzymes (although the TTTN + 20bp version of Cpf1 is currently supported); see issue #13. I'm working on a version that supports arbitrary patterns which should be ready sometime soon. Thanks for your interest!