This repository contains data for our polymerase error quantification protocol. This protocol relies on unique molecular identifier (UMI) technique and a sampling bottleneck to ensure elimination library preparation and sequencing errors while retaining original PCR error profile. Initial data analysis is performed using MAGERI software, see processing/ folder in this repository for configuration files and shell script.
All figures reported in our study can be generated by compiling an R markdown template polyfid.Rmd, the template can be viewed and compiled using RStudio.
The resulting PDF can be found here.