mikolmogorov/Flye

What parameter for R10.4.1 FAST mode data

LogCrab opened this issue · 1 comments

Hi, developers of Flye. Recently I acquired some R10.4.1 sequencing data. But unfortunately, these data is sequenced by unprofessional technician in the hospital, who only saved the FAST basecalled data from MinKnow. I wonder what parameter should I use for achieving the best performance in Flye. Should it be --nano-raw since FAST mode contain many sequencing error?

Hello, as long as error rate is below 3-4%, you can still use --nano-hq