muyifang1's Stars
huangjia2019/chatgpt-briefing
极客时间 - ChatGPT公开课
chatanywhere/GPT_API_free
Free ChatGPT API Key，免费ChatGPT API，支持GPT4 API（免费），ChatGPT国内可用免费转发API，直连无需代理。可以搭配ChatBox等软件/插件使用，极大降低接口使用成本。国内即可无限制畅快聊天。
aandrew-me/tgpt
AI Chatbots in terminal without needing API keys
apache/superset
Apache Superset is a Data Visualization and Data Exploration Platform
hyper0x/Golang_Puzzlers
An example project, for my column named "Core Golang - 36 lessons"
zsnmwy/golang
kubernetes/kubernetes
Production-Grade Container Scheduling and Management
Alvin9999/new-pac
翻墙-科学上网、自由上网、免费科学上网、免费翻墙、油管youtube、fanqiang、软件、VPN、一键翻墙浏览器，vps一键搭建翻墙服务器脚本/教程，免费shadowsocks/ss/ssr/v2ray/goflyway账号/节点，翻墙梯子，电脑、手机、iOS、安卓、windows、Mac、Linux、路由器翻墙、科学上网、youtube视频下载、美区apple id共享账号
zsnmwy/101
cncamp/101
A basic instruction for Kubernetes setup and understanding.
flycash/toy-web
用于极客时间go基础课程
lindongchen/drag-topology-editor-vue3
This is a editor for topology dragable (vue 3.0)
CaiBaoHong/biu
管理后台项目开发脚手架，基于vue-element-admin和springboot搭建，前后端分离方式开发和部署
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
sopify-bot/nikebot
nikebot nike抢鞋 NIKE AKAMAI BOT NIKE监控 nike补货监控 nike抽签 snrks抢购 nike抢购 snkrs抽签 nike发包程序 nike发包抢购 nike破盾
apache/dubbo
The java implementation of Apache Dubbo. An RPC and microservice framework.
sdlpal/sdlpal
SDL-based reimplementation of the classic Chinese-language RPG known as PAL.
AliyunContainerService/k8s-for-docker-desktop
为Docker Desktop for Mac/Windows开启Kubernetes和Istio。
modelmapper/modelmapper
Intelligent object mapping
geektime-geekbang/staffjoy
微服务和云原生架构教学案例项目，基于Spring Boot和Kubernetes技术栈
dromara/hmily
Distributed transaction solutions
apache/shardingsphere
Distributed SQL transaction & query engine for data sharding, scaling, encryption, and more - on any database.
bjmashibing/java
zookeeperss/JavaGuide
【Java学习+面试指南】 一份涵盖大部分Java程序员所需要掌握的核心知识。
geektime-geekbang/LetsJava
JavaCourse00/JavaCourseCodes
JavaCourse
apache/shenyu
Apache ShenYu is a Java native API Gateway for service proxy, protocol conversion and API governance.
alibaba/druid
阿里云计算平台DataWorks(https://help.aliyun.com/document_detail/137663.html) 团队出品，为监控而生的数据库连接池
geektime-geekbang/geektime-spring-family
极客时间视频课程《玩转Spring全家桶》
cncounter/translation
翻译文档