Project: Study of a microglia-specific eQTL

Authors: Rebekah Langston and J. Raph Gibbs

This repository contains scripts used to produce the results presented in our manuscript titled "Association of a Common Genetic Variant with Parkinson’s Disease is Propagated through Microglia".

These datasets are analyzed:

  • Single nuclei RNA sequencing of human frontal cortex
  • Single cell RNA sequencing of iPSC-derived microglia
  • Target mRNA and protein expression in iPSC-derived microglia
  • Single nuclei ATAC sequencing of human frontal cortex
  • Single nuclei Multiome (GEX + ATAC) sequencing of human substantia nigra
    • Also analysis of publicly available human substantia nigra snRNAseq datasets
  • Bulk ATAC sequencing of iPSC-derived cells
    • Also integration of bulk ATACseq and snATACseq datasets
  • Single cell CRISPRi screen in iPSC-derived microglia targeting LRRK2 CREs