/midnight-artic-1200

primers and bed files associated with the Midnight ARTIC 1200 bp primer scheme for SARS-CoV-2 sequencing

GNU General Public License v3.0GPL-3.0

midnight-artic-1200

These are the primer sets and bed files associated with the Midnight ARTIC 1200 bp primer scheme for SARS-CoV-2 sequencing. v1 is the first version as published in Freed et al. 2020. This work arose from the foundational work done by the ARTIC Netwwork.

v1.1 is the first improvement to the Midnight scheme, due to a theoretical proposal by Nikki Freed and myself, with confirmation by Oxford Nanopore. This fixes the amplicon 28 dropout seen in Omicron, and as of January 2022 is the design used by Oxford Nanopore.

v1.2 is the second improvement, due to work by John Tyson at the British Columbia Centre for Disease Control. This includes three additional primers as compared to v1.1, which fixes coverage decreases seen in Omicron. As of February 2022 this will be the pool released by IDT.