sequencing-tricks

#Allow more than 0 mismatch when filtering the sequence data, although you shouldn't set more than 3 mismatch since the total bar code is 6 nuclear tide.

bar_code_filter.py

#This code is designed for experimentist working through biowulf (NIH HPC) to automatically download sra file (SRRXXXXX) and align them to mouse genome using STAR.

download_sample_and_alignment_PE.py

#This is a basic filter of vcf files. You have to install vcftools, bcftools and vcffilter first.

vcf_filter.sh