/foxmap

Scripts for Chromosome Assembly of the Red Fox

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Scripts for Chromosome Assembly of the Red Fox

These are the essential scripts developed in the assembly of the red fox genome. They are made available here in case they can be useful for future analyses.

essential_mods.py is a set of very simple functions that we used to pull sequence from the scaffolds and orient it appropriately.
racaout_to_csv.py takes RACA's output files and transforms them to a csv format that is easier to use for analysis (in Excel, Python, R, etc.)
make-frags.py takes the scaffolds and the RACA input to make the fasta files that comprise 1) RACA's assembly, and 2) the chromosome fragment assembly. It depends on:

  1. A comma-separated file (here: racafrag_foxfrag.csv) that specifies the relationship between RACA fragments and fox fragments. It shoudl have a header (Fragment,FoxSeg) and each line should list the RACA Fragment and its associated Chromosomal Fragment (e.g. 26a,10
  2. Syntenic relationships between the dog (reference) and fox (target) species, which are hard-coded into the script here as a list (line 113)
  3. the essential_mods.py functions
  4. RACA's output
  5. The scaffolds

You can reach out to me at rando2 at Illinois dot edu if you have any questions.

MIT License

Copyright (c) [2018] [Halie M. Rando]

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