/BIO331L_Molecular_Biology_Lab

Investigate the effects of knocking out Arabinogalactan protein 31 on CRU3 and OLE1 protein and mRNA expression in Arabidopsis thaliana seedlings.

Primary LanguageR

BIO331L: Lab Studies in Molecular Biology

Overview

We investigate the effects of knocking out Arabinogalactan protein 31 on Cruciferin 3 protein expression, Cruciferin 3 mRNA expression, and Oleosin mRNA expression in Arabidopsis thaliana seedlings.


Molecular techniques

Report 1

  • Protein purification
  • Bradford Assay
  • SDS-PAGE
  • Western Blot

Report 2

  • RNA purification
  • cDNA library preparation
  • Primer standardization
  • RT-qPCR