recep-yildiz's Stars
tabler/tabler
Tabler is free and open-source HTML Dashboard UI Kit built on Bootstrap
electron/electron-api-demos
Explore the Electron APIs
idea4good/GuiLite
✔️The smallest header-only GUI library(4 KLOC) for all platforms
laravel/jetstream
Tailwind scaffolding for the Laravel framework.
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
googlearchive/android-ConstraintLayoutExamples
Migrated:
mirari/v-viewer
Image viewer component for vue, supports rotation, scale, zoom and so on, based on viewer.js
google-research-datasets/Objectron
Objectron is a dataset of short, object-centric video clips. In addition, the videos also contain AR session metadata including camera poses, sparse point-clouds and planes. In each video, the camera moves around and above the object and captures it from different views. Each object is annotated with a 3D bounding box. The 3D bounding box describes the object’s position, orientation, and dimensions. The dataset contains about 15K annotated video clips and 4M annotated images in the following categories: bikes, books, bottles, cameras, cereal boxes, chairs, cups, laptops, and shoes
chenxuan0000/vue-seamless-scroll
:beginner:A simple, seamless scrolling for Vue.js vue无缝滚动component
chronotruck/vue-ctk-date-time-picker
VueJS component to select dates & time, including a range mode
OneWayTech/vue2-datatable
(DEPRECATED) The best Datatable for Vue.js 2.x which never sucks. Give us a star 🌟 if you like it!
EverexIO/Ethplorer
Viewer of Ethereum tokens and transactions based on ERC20 standard.
cosmic-byte/flask-restplus-boilerplate
A boilerplate for flask restful web service
gro-ove/actools
Alternative launcher for Assetto Corsa named Content Manager, and some utils as well.
softwareQinc/qpp
Modern C++ quantum computing library
SocketCluster/sc-crud-sample
Sample real-time CRUD inventory tracking app built with SocketCluster
inorganik/debugout.js
Generates a text file from your logs.
chriszarate/bookmarkleter
You have JavaScript. You need a bookmarklet. This does that.
TerryZ/v-uploader
A Vue2 plugin make files upload simple and easier, single file upload with image preview, multiple upload with drag and drop
Dominique92/ol-geocoder
Geocoder Nominatim for OpenLayers
nhn/toast-ui.vue-image-editor
Toast UI Image Editor for Vue
eregnier/vue2-gmap-custom-marker
vue google map custom marker component
lekhang2512/vue-upload-multiple-image
A simple upload multiple image component for Vuejs
andrechen/yuv2rgb
transform yuv to rgb
soundar24/vue-round-slider
A round slider component for Vue JS, with range slider support. Also it can be any kind of arc slider such as pie shape, half / semi-circle, quarter circle shape sliders.
idea4good/GuiLiteWeb
🎲GuiLite 3D research
emreturan/heikin-ashi
Heikin-Ashi candlesticks for pandas dataframe.
fatmakiraz/personal-blog-template
Personal Blog Template is a HTML/ CSS template including hero, works and blog sections prepared by using Sass/SCSS and LiquidJS.
Ethereum-Max/EMAX-Contracts
Contracts for the EMAX token on Ethereum Mainnet
fatmakiraz/product-checkout-e-commerce-template
Product & Checkout E-Commerce Template is an HTML/ CSS template including some pages which are product, cart, address, shipping and payment detail pages.