ENCODE Transcription Factor and Histone ChIP-Seq processing pipeline

Conda environment name change (since v2.2.0 or 6/13/2022)

Pipeline's Conda environment's names have been shortened to work around the following error:

PaddingError: Placeholder of length '80' too short in package /XXXXXXXXXXX/miniconda3/envs/

You need to reinstall pipeline's Conda environment. It's recommended to do this for every version update.

$ bash scripts/uninstall_conda_env.sh
$ bash scripts/install_conda_env.sh

Introduction

This ChIP-Seq pipeline is based off the ENCODE (phase-3) transcription factor and histone ChIP-seq pipeline specifications (by Anshul Kundaje) in this google doc.

Features

Portability: The pipeline run can be performed across different cloud platforms such as Google, AWS and DNAnexus, as well as on cluster engines such as SLURM, SGE and PBS.
User-friendly HTML report: In addition to the standard outputs, the pipeline generates an HTML report that consists of a tabular representation of quality metrics including alignment/peak statistics and FRiP along with many useful plots (IDR/cross-correlation measures). An example of the HTML report. The json file used in generating this report.
Supported genomes: Pipeline needs genome specific data such as aligner indices, chromosome sizes file and blacklist. We provide a genome database downloader/builder for hg38, hg19, mm10, mm9. You can also use this builder to build genome database from FASTA for your custom genome.

Installation

Make sure that you have Python>=3.6. Caper does not work with Python2. Install Caper and check its version >=2.0.

$ pip install caper

# use caper version >= 2.3.0 for a new HPC feature (caper hpc submit/list/abort).
$ caper -v

Read Caper's README carefully to choose a backend for your system. Follow the instruction in the configuration file.

# this will overwrite the existing conf file ~/.caper/default.conf
# make a backup of it first if needed
$ caper init [YOUR_BACKEND]

# edit the conf file
$ vi ~/.caper/default.conf

Git clone this pipeline.

$ cd
$ git clone https://github.com/ENCODE-DCC/chip-seq-pipeline2

(Optional for Conda) DO NOT USE A SHARED CONDA. INSTALL YOUR OWN MINICONDA3 AND USE IT. Install pipeline's Conda environments if you don't have Singularity or Docker installed on your system. We recommend to use Singularity instead of Conda.

# check if you have Singularity on your system, if so then it's not recommended to use Conda
$ singularity --version

# check if you are not using a shared conda, if so then delete it or remove it from your PATH
$ which conda

# change directory to pipeline's git repo
$ cd chip-seq-pipeline2

# uninstall old environments
$ bash scripts/uninstall_conda_env.sh

# install new envs, you need to run this for every pipeline version update.
# it may be killed if you run this command line on a login node.
# it's recommended to make an interactive node and run it there.
$ bash scripts/install_conda_env.sh

Input JSON file

IMPORTANT: DO NOT BLINDLY USE A TEMPLATE/EXAMPLE INPUT JSON. READ THROUGH THE FOLLOWING GUIDE TO MAKE A CORRECT INPUT JSON FILE.

An input JSON file specifies all the input parameters and files that are necessary for successfully running this pipeline. This includes a specification of the path to the genome reference files and the raw data fastq file. Please make sure to specify absolute paths rather than relative paths in your input JSON files.

Running on local computer/HPCs

You can use URIs(s3://, gs:// and http(s)://) in Caper's command lines and input JSON file then Caper will automatically download/localize such files. Input JSON file example: https://storage.googleapis.com/encode-pipeline-test-samples/encode-chip-seq-pipeline/ENCSR000DYI_subsampled_chr19_only.json

According to your chosen platform of Caper, run Caper or submit Caper command line to the cluster. You can choose other environments like --singularity or --docker instead of --conda. But you must define one of the environments.

PLEASE READ CAPER'S README VERY CAREFULLY BEFORE RUNNING ANY PIPELINES. YOU WILL NEED TO CORRECTLY CONFIGURE CAPER FIRST. These are just example command lines.

```bash
# Run it locally with Conda (DO NOT ACTIVATE PIPELINE'S CONDA ENVIRONEMT)
$ caper run chip.wdl -i https://storage.googleapis.com/encode-pipeline-test-samples/encode-chip-seq-pipeline/ENCSR000DYI_subsampled_chr19_only.json --conda

# On HPC, submit it as a leader job to SLURM with Singularity
$ caper hpc submit chip.wdl -i https://storage.googleapis.com/encode-pipeline-test-samples/encode-chip-seq-pipeline/ENCSR000DYI_subsampled_chr19_only.json --singularity --leader-job-name ANY_GOOD_LEADER_JOB_NAME

# Check job ID and status of your leader jobs
$ caper hpc list

# Cancel the leader node to close all of its children jobs
# If you directly use cluster command like scancel or qdel then
# child jobs will not be terminated
$ caper hpc abort [JOB_ID]
```

Running on Terra/Anvil (using Dockstore)

Visit our pipeline repo on Dockstore. Click on Terra or Anvil. Follow Terra's instruction to create a workspace on Terra and add Terra's billing bot to your Google Cloud account.

Download this test input JSON for Terra and upload it to Terra's UI and then run analysis.

If you want to use your own input JSON file, then make sure that all files in the input JSON are on a Google Cloud Storage bucket (gs://). URLs will not work.

Running on DNAnexus (using Dockstore)

Sign up for a new account on DNAnexus and create a new project on either AWS or Azure. Visit our pipeline repo on Dockstore. Click on DNAnexus. Choose a destination directory on your DNAnexus project. Click on Submit and visit DNAnexus. This will submit a conversion job so that you can check status of it on Monitor on DNAnexus UI.

Once conversion is done download one of the following input JSON files according to your chosen platform (AWS or Azure) for your DNAnexus project:

You cannot use these input JSON files directly. Go to the destination directory on DNAnexus and click on the converted workflow chip. You will see input file boxes in the left-hand side of the task graph. Expand it and define FASTQs (fastq_repX_R1 and fastq_repX_R1) and genome_tsv as in the downloaded input JSON file. Click on the common task box and define other non-file pipeline parameters. e.g. pipeline_type, paired_end and ctl_paired_end.

We have a separate project on DNANexus to provide example FASTQs and genome_tsv for hg38 and mm10 (also chr19-only version of those two. Use chr19-only versions for testing). We recommend to make copies of these directories on your own project.

genome_tsv

Example FASTQs

Running on DNAnexus (using our pre-built workflows)

See this for details.

Running and sharing on Truwl

You can run this pipeline on truwl.com. This provides a web interface that allows you to define inputs and parameters, run the job on GCP, and monitor progress. To run it you will need to create an account on the platform then request early access by emailing info@truwl.com to get the right permissions. You can see the example cases from this repo at https://truwl.com/workflows/instance/WF_dd6938.8f.340f/command and https://truwl.com/workflows/instance/WF_dd6938.8f.8aa3/command. The example jobs (or other jobs) can be forked to pre-populate the inputs for your own job.

If you do not run the pipeline on Truwl, you can still share your use-case/job on the platform by getting in touch at info@truwl.com and providing your inputs.json file.

How to organize outputs

Install Croo. You can skip this installation if you have installed pipeline's Conda environment and activated it. Make sure that you have python3(> 3.4.1) installed on your system. Find a metadata.json on Caper's output directory.

$ pip install croo
$ croo [METADATA_JSON_FILE]

How to make a spreadsheet of QC metrics

Install qc2tsv. Make sure that you have python3(> 3.4.1) installed on your system.

Once you have organized output with Croo, you will be able to find pipeline's final output file qc/qc.json which has all QC metrics in it. Simply feed qc2tsv with multiple qc.json files. It can take various URIs like local path, gs:// and s3://.

$ pip install qc2tsv
$ qc2tsv /sample1/qc.json gs://sample2/qc.json s3://sample3/qc.json ... > spreadsheet.tsv

QC metrics for each experiment (qc.json) will be split into multiple rows (1 for overall experiment + 1 for each bio replicate) in a spreadsheet.

Troubleshooting

See this document for troubleshooting. I will keep updating this document for errors reported by users.

sidwekhande/chip-seq-pipeline2