Unable to open files in mzR if ignoreInstrumentError flag used in msconvert
wilsontom opened this issue · 11 comments
wilsontom commented
When using the --ignoreInstrumentError flag in msconvert the converted mzML files are not readable by mzR
library(mzR)
openMSfile('Calcifediol_P.mzML')
Error: Can not open file Calcifediol_P.mzML! Original error was:
Error in pwizModule$open(filename): [IO::HandlerBinaryDataArray] Unknown binary data type.sessionInfo()
> sessionInfo()
R version 4.0.5 (2021-03-31)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.10
Matrix products: default
BLAS: /usr/lib/x86_64-linux-gnu/blas/libblas.so.3.9.0
LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.9.0
locale:
[1] LC_CTYPE=en_GB.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_GB.UTF-8 LC_COLLATE=en_GB.UTF-8
[5] LC_MONETARY=en_GB.UTF-8 LC_MESSAGES=en_GB.UTF-8
[7] LC_PAPER=en_GB.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_GB.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] mzR_2.24.1 Rcpp_1.0.6
loaded via a namespace (and not attached):
[1] compiler_4.0.5 ProtGenerics_1.22.0 parallel_4.0.5
[4] Biobase_2.50.0 codetools_0.2-18 ncdf4_1.17
[7] BiocGenerics_0.36.1sneumann commented
Hi Tom, thanks for the report. Could you provide a cut-down example here ? E.g. open in your favourite editor and delete all but the first handful of spectra ? Also a reminder what --ignoreInstrumentError does. Yours, Steffen
wilsontom commented
Hi Steffan,
For some older instruments (in this case a Thermo TSQ Quantum Ultra) msconvert throws an error because it can't parse the instrument model ([Reader_Thermo::fillInMetadata] unable to parse instrument model), adding the --ignoreInstrumentError overrides this to allow conversion.
This is an extract of an mzML file that does open ok in mzR (converted using msconvert via WindowsOS command prompt, ProteoWizard release: 3.0.10246 (2016-12-5))
<indexedmzML xmlns="http://psi.hupo.org/ms/mzml" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/ms/mzml http://psidev.info/files/ms/mzML/xsd/mzML1.1.2_idx.xsd">
<mzML xmlns="http://psi.hupo.org/ms/mzml" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/ms/mzml http://psidev.info/files/ms/mzML/xsd/mzML1.1.0.xsd" id="Cyanocobalamin_P" version="1.1.0">
<cvList count="2">
<cv id="MS" fullName="Proteomics Standards Initiative Mass Spectrometry Ontology" version="4.0.1" URI="http://psidev.cvs.sourceforge.net/*checkout*/psidev/psi/psi-ms/mzML/controlledVocabulary/psi-ms.obo"/>
<cv id="UO" fullName="Unit Ontology" version="12:10:2011" URI="http://obo.cvs.sourceforge.net/*checkout*/obo/obo/ontology/phenotype/unit.obo"/>
</cvList>
<fileDescription>
<fileContent>
<cvParam cvRef="MS" accession="MS:1001473" name="selected reaction monitoring chromatogram" value=""/>
</fileContent>
<sourceFileList count="1">
<sourceFile id="RAW1" name="Cyanocobalamin_P.RAW" location="file:///">
<cvParam cvRef="MS" accession="MS:1000768" name="Thermo nativeID format" value=""/>
<cvParam cvRef="MS" accession="MS:1000563" name="Thermo RAW format" value=""/>
<cvParam cvRef="MS" accession="MS:1000569" name="SHA-1" value="a0a9ded6f1e72c5bb92b31668110652163626ef2"/>
</sourceFile>
</sourceFileList>
</fileDescription>
<referenceableParamGroupList count="1">
<referenceableParamGroup id="CommonInstrumentParams">
<cvParam cvRef="MS" accession="MS:1000492" name="Thermo Electron instrument model" value=""/>
<cvParam cvRef="MS" accession="MS:1000529" name="instrument serial number" value="TQU01681"/>
<userParam name="instrument model" value="TSQ Quantum Ultra EMR"/>
</referenceableParamGroup>
</referenceableParamGroupList>
<softwareList count="2">
<software id="Xcalibur" version="1.5 SP 1">
<cvParam cvRef="MS" accession="MS:1000532" name="Xcalibur" value=""/>
</software>
<software id="pwiz" version="3.0.10235">
<cvParam cvRef="MS" accession="MS:1000615" name="ProteoWizard software" value=""/>
</software>
</softwareList>
<instrumentConfigurationList count="1">
<instrumentConfiguration id="IC">
<cvParam cvRef="MS" accession="MS:1000031" name="instrument model" value=""/>
</instrumentConfiguration>
</instrumentConfigurationList>
<dataProcessingList count="1">
<dataProcessing id="pwiz_Reader_Thermo_conversion">
<processingMethod order="0" softwareRef="pwiz">
<cvParam cvRef="MS" accession="MS:1000544" name="Conversion to mzML" value=""/>
</processingMethod>
</dataProcessing>
</dataProcessingList>
<run id="Cyanocobalamin_P" defaultInstrumentConfigurationRef="IC" startTimeStamp="2021-04-27T15:36:23Z" defaultSourceFileRef="RAW1">
<chromatogramList count="50" defaultDataProcessingRef="pwiz_Reader_Thermo_conversion">
<chromatogram index="0" id="TIC" defaultArrayLength="1903">
<cvParam cvRef="MS" accession="MS:1000235" name="total ion current chromatogram" value=""/>
<binaryDataArrayList count="2">
<binaryDataArray encodedLength="20300">
<cvParam cvRef="MS" accession="MS:1000523" name="64-bit float" value=""/>
<cvParam cvRef="MS" accession="MS:1000576" name="no compression" value=""/>
<cvParam cvRef="MS" accession="MS:1000595" name="time array" value="" unitCvRef="UO" unitAccession="UO:0000031" unitName="minute"/>
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This is an extract from an mzML file (of the same RAW file) which doesn't open in mzR. This has been converted using the pwiz docker container (ProteoWizard release: 3.0.20287 (769529fa4))
<?xml version="1.0" encoding="utf-8"?>
<indexedmzML xmlns="http://psi.hupo.org/ms/mzml" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/ms/mzml http://psidev.info/files/ms/mzML/xsd/mzML1.1.2_idx.xsd">
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<cv id="MS" fullName="Proteomics Standards Initiative Mass Spectrometry Ontology" version="4.1.41" URI="https://raw.githubusercontent.com/HUPO-PSI/psi-ms-CV/master/psi-ms.obo"/>
<cv id="UO" fullName="Unit Ontology" version="09:04:2014" URI="https://raw.githubusercontent.com/bio-ontology-research-group/unit-ontology/master/unit.obo"/>
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<fileContent>
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<userParam name="instrument model" value="TSQ Quantum Ultra EMR"/>
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</referenceableParamGroupList>
<sampleList count="1">
<sample id="_x0034_" name="">
<cvParam cvRef="MS" accession="MS:1000002" name="sample name" value="4"/>
</sample>
</sampleList>
<softwareList count="2">
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<cvParam cvRef="MS" accession="MS:1000532" name="Xcalibur" value=""/>
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<software id="pwiz" version="3.0.20287">
<cvParam cvRef="MS" accession="MS:1000615" name="ProteoWizard software" value=""/>
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</softwareList>
<instrumentConfigurationList count="1">
<instrumentConfiguration id="IC1">
<referenceableParamGroupRef ref="CommonInstrumentParams"/>
<componentList count="1">
<source order="1">
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</instrumentConfiguration>
</instrumentConfigurationList>
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<cvParam cvRef="MS" accession="MS:1000576" name="no compression" value=""/>
<cvParam cvRef="MS" accession="MS:1000786" name="non-standard data array" value="ms level" unitCvRef="UO" unitAccession="UO:0000186" unitName="dimensionless unit"/>
<binary>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                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                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  </binary>
</binaryDataArray>
</binaryDataArrayList>
</chromatogram>
<chromatogram index="1" id="- SRM SIC 117.087,55.033" defaultArrayLength="173">
<cvParam cvRef="MS" accession="MS:1001473" name="selected reaction monitoring chromatogram" value=""/>
<cvParam cvRef="MS" accession="MS:1000129" name="negative scan" value=""/>
<precursor>
<isolationWindow>
<cvParam cvRef="MS" accession="MS:1000827" name="isolation window target m/z" value="117.087" unitCvRef="MS" unitAccession="MS:1000040" unitName="m/z"/>
</isolationWindow>
<activation>
<cvParam cvRef="MS" accession="MS:1000133" name="collision-induced dissociation" value=""/>
<cvParam cvRef="MS" accession="MS:1000045" name="collision energy" value="0.0"/>
</activation>
</precursor>
<product>
<isolationWindow>
<cvParam cvRef="MS" accession="MS:1000827" name="isolation window target m/z" value="55.033" unitCvRef="MS" unitAccession="MS:1000040" unitName="m/z"/>
<cvParam cvRef="MS" accession="MS:1000828" name="isolation window lower offset" value="0.005" unitCvRef="MS" unitAccession="MS:1000040" unitName="m/z"/>
<cvParam cvRef="MS" accession="MS:1000829" name="isolation window upper offset" value="0.005" unitCvRef="MS" unitAccession="MS:1000040" unitName="m/z"/>
</isolationWindow>
</product>
<binaryDataArrayList count="2">
<binaryDataArray encodedLength="1848">
<cvParam cvRef="MS" accession="MS:1000523" name="64-bit float" value=""/>
<cvParam cvRef="MS" accession="MS:1000576" name="no compression" value=""/>
<cvParam cvRef="MS" accession="MS:1000595" name="time array" value="" unitCvRef="UO" unitAccession="UO:0000031" unitName="minute"/>
<binary>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</binary>
</binaryDataArray>
<binaryDataArray encodedLength="924">
<cvParam cvRef="MS" accession="MS:1000521" name="32-bit float" value=""/>
<cvParam cvRef="MS" accession="MS:1000576" name="no compression" value=""/>
<cvParam cvRef="MS" accession="MS:1000515" name="intensity array" value="" unitCvRef="MS" unitAccession="MS:1000131" unitName="number of detector counts"/>
<binary>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</binary>
</binaryDataArray>
</binaryDataArrayList>
From what I can see, the key difference between the files is <instrumentConfigurationList count="1">
This is the block from the working file
<instrumentConfigurationList count="1">
<instrumentConfiguration id="IC">
<cvParam cvRef="MS" accession="MS:1000031" name="instrument model" value=""/>
</instrumentConfiguration>
</instrumentConfigurationList>
This is the block from the file that doesn't open
<instrumentConfigurationList count="1">
<instrumentConfiguration id="IC1">
<referenceableParamGroupRef ref="CommonInstrumentParams"/>
<componentList count="1">
<source order="1">
<cvParam cvRef="MS" accession="MS:1000073" name="electrospray ionization" value=""/>
<cvParam cvRef="MS" accession="MS:1000057" name="electrospray inlet" value=""/>
</source>
</componentList>
<softwareRef ref="Xcalibur"/>
</instrumentConfiguration>
</instrumentConfigurationList>
Thanks
Tom
lgatto commented
A temporary fix that I sometimes apply when a CV param isn't recognised by mzR (such as MS:1003145 below) is to remove the offending line with something like
perl -pi -e 's/^.+MS:1003145.+\n$//' raw.mzML
sneumann commented
Hi, given the Unknown binary data type I'd expect the CV terms for instruments are mostly known,
and it is rather concerning the <binaryDataArray> tags. Before diving into
mzR/src/pwiz/data/msdata/IO.cpp
Line 1702 in 022ea55
I'd like to see where exactly in the mzML the culprit is. When I said cut-down I was a bit imprecise, can you provide an mzML as attachment. I have an inkling that it is the
"non-standard data array" value="ms level" that confuses the code.Yours,
Steffen
wilsontom commented
Hi Steffan,
I've included the two mzML files (as .txt)
Cyanocobalamin_NotWorking .txt
Cyanocobalamin_Working.txt
attached
Thanks
Tom
sneumann commented
Ok, so as a start you probably found an issue with pwiz:
mscat Cyanocobalamin_NotWorking.mzML ## libpwiz-tools 3.0.18342-2
[IO::HandlerBinaryDataArray] Unknown binary data type.
And indeed removing the
<binaryDataArray arrayLength="1903" encodedLength="20300">
<cvParam cvRef="MS" accession="MS:1000522" name="64-bit integer" value=""/>
<cvParam cvRef="MS" accession="MS:1000576" name="no compression" value=""/>
<cvParam cvRef="MS" accession="MS:1000786" name="non-standard data array" value="ms level" unitCvRef="UO" unitAccession="UO:0000186" unitName="dimensionless unit"/>
<binary>AgAAAAA....</binary>
</binaryDataArray>
makes mscat (and mzR) happy. I suggest you file a bug at https://github.com/ProteoWizard/pwiz/issues or even provide a PR there. Then mzR can follow suit.
Yours,
Steffen
wilsontom commented
Hi Steffan,
Thanks very much for looking into this, much appreciated.
I'll raise the issue with pwiz, and take it from there
Thanks
Tom
joerg-b commented
Good evening, I found this thread looking for the same error message.
I've been working on files that I've downloaded from Metabolights (MTBLS429). When I convert the .raw files to .mzML using ProteoWizard 3.0.10158 I can read them using mzR without problem. However if I use ProteoWizard 3.0.21193 I get the same error message that wilsontom has seen.
Looking at this thread
https://skyline.ms/announcements/home/support/thread.view?rowId=48705
I get the impression that a feature that the ProteoWizard people added on purpose is causing the problem.
Of course I could go back to the old ProteoWizard version that I have, however I'm working on a different data set (MTBLS897) that causes the old ProteoWizard to crash and that can be converted by the newer version. Unfortunately I couldn't get hold of an in-between version.
Best
Joerg
P.S.
the problem seems to arise from the 3rd block of binary data in chromatogram 0 (TIC) that the newer version of ProteoWizard is generating:
<binaryDataArray arrayLength="1735" encodedLength="52">
<cvParam cvRef="MS" accession="MS:1000519" name="32-bit integer" value=""/>
<cvParam cvRef="MS" accession="MS:1000574" name="zlib compression" value=""/>
<cvParam cvRef="MS" accession="MS:1000786" name="non-standard data array" value="function" unitCvRef="UO" unitAccession="UO:0000186" unitName="dimensionless unit"/>
<binary>eJztxTERAAAIBKBX+3fWHjJwVJI+Zdu2bdsPHtu2bdv26xdutgvu</binary>
</binaryDataArray>
If I remove these lines manually and change in this line
the number value to "2", then I can read the file with mzR.
sneumann commented
That adds pressure to update the embedded pwiz. I have tried a few times and each time failed horribly :-(
sneumann commented
Hi, could people check if mzR >= 2.29.3 fixes their issues, so we can close in case of success ?
I have a working
> m <- openMSfile("Cyanocobalamin_NotWorking.mzML")
> m
Mass Spectrometry file handle.
Filename: Cyanocobalamin_NotWorking.mzML
Number of scans: 0
Yours, Steffen
wilsontom commented
Hi Steffan,
Just tested with the current GH version, and all working OK !
Thanks for getting this pwiz update sorted
Tom