Use proteases specificity
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douweschulte commented
Per read it is known which protease generated the peptide, this can be used in the assignment of hard amino acids. Chymotrypsin is known to have specificity after I/L. But a simple search with a sample dataset using database matching gave the impression that this specificity is not 100%. TO actually implement this a more accurate idea on the specificity is needed, after that these specificaties can be weighed in into the consensus sequence.