/Age-Realted_CpGs_ZF_JD

Code for DNA methylation extraction, age-related CpG site identification, data manipulation, genomic annotation, statistical analyses and plots include in submitted paper on sex chromosome-mediated aging methylomes in female heterogametic species

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title authors date
Identification of age-related CpG sites from longitudinal DNA methylation data of two avian species
Marianthi Tangili, Joanna Sudyka, Fabricio Furni
24/04/2024

Data for identification of age-related CpG sites from longitudinal avian methylomes

01: Bioinformatics code for DNA methylation extraction from WGBS and EMSeq data

02: Merging DNA methylation measurements per CpG site per sample in one data frame per species
Running the initial part of the pipeline for identification of age-related CpG sites

03: Functional annotation of all CpG sites captured by our analysis

04: Final steps of the pipeline
Functional annotation of age-related CpG sites
Statistical analyses
Plots

preprint: https://www.biorxiv.org/content/10.1101/2024.07.31.605993v1

Information about each step is found as comments in the scripts

Scripts 01,02,03 were run using the hpc of the University of Groningen Hábrók (https://www.rug.nl/society-business/centre-for-information-technology/research/services/hpc/facilities/habrok-hpc-cluster?lang=en)

The raw sequencing data used in this paper (for both species) can be found at: https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1108628