wdecoster/nanostat

Barcoded output not available

Closed this issue · 4 comments

Hello Wouter,

Thank you for creating this amazing tool for Nanopore statistics. I am trying to get the barcoded statistics from a sequencing_summary.txt

The command I use :-
NanoStat --threads 20 --barcoded --tsv --summary sequencing_summary.txt -o example -n 4a

When I use, the above command I don't find a output NanoStat_Barcoded.txt (seen in NanoPlot) or in the result out 4a - I don't see any barcoded statistics either, rather I see the total statistics of the run. However, when I print it to stdout, I see the results of barcoded statistics (in wide format).

Is there something wrong in my command or could this be a possible bug?

Tried the above using NanoStats v1.4.0 and v1.5.0.

Thank you,
-s

Thanks for reporting this! I identified the cause, and I will have a fix online soon.

Wouter

This should be solved in NanoStat v1.6.0, which is available through pip now and through conda soon

Hello,
I had the same issue which has been resolved using the last version 1.6.0. However now the start of the output file (general summary to Total bases lines) are not separated by tab between barcoded samples. It is normal? I used the command --barcoded --summary -o -n and --tsv.

Thanks for the help.