could not find function, 'norm.to.one'
Ci-TJ opened this issue · 1 comments
Ci-TJ commented
Hi! I just installed TED, but I couldn't find function, norm.to.one.
> install_github("Danko-Lab/TED/TED")
Downloading GitHub repo Danko-Lab/TED@HEAD
Error in utils::download.file(url, path, method = method, quiet = quiet, :
download from 'https://api.github.com/repos/Danko-Lab/TED/tarball/HEAD' failed
## I download the package from GitHub, and install it locally.
> TED::
TED::learn.embedding.withPhiTum TED::learn.embedding.Kcls TED::run.Ted TED::cleanup.genes TED::estimate_sf TED::get.signature.genes TED::convert.cell.fraction
>
> TED::norm.to.one
Error: 'norm.to.one' is not an exported object from 'namespace:TED'
> help(package="TED")
Information on package ‘TED’
Description:
Package: TED
Version: 1.1
Date: 2020-01-15
Title: BayesPrism: A Fully Bayesian Inference of Tumor
Microenvironment composition and gene expression.
Formerly called TED (Tumor microEnvironment
Deconvolution).
Author: Tinyi Chu<tc532@cornell.edu>, Charles G. Danko
<dankoc@gmail.com>
Maintainer: Tinyi Chu<tc532@cornell.edu>
Depends: R (>= 2.6)
Imports: DESeq2, parallel, MCMCpack, gplots, scran, BiocParallel
Description: TED is comprised of the deconvolution modules and the
embedding learning module. The deconvolution module
leverages cell type-specific expression profiles from
scRNA-seq and implements a fully Bayesian inference to
jointly estimate the posterior distribution of cell type
composition and cell type-specific gene expression from
bulk RNA-seq expression of tumor samples. The embedding
learning module uses Expectation-maximization (EM) to
approximate the tumor expression using a linear
combination of tumor pathways while conditional on the
inferred expression and fraction of non-tumor cells
estimated by the deconvolution module.
License: GPL-2 | GPL-3
biocViews: Sequencing, Analysis
LazyLoad: yes
RoxygenNote: 6.0.1
RemoteType: local
RemoteUrl: /home/user_li/linqin_tmp/SourceCode/ENIGMA/TED.zip
Built: R 4.0.3; ; 2021-12-26 11:38:32 UTC; unix
Index:
cleanup.genes Utility function to remove highly expressed
outlier genes that are sensitive to batch
effects from ref.dat
learn.embedding.Kcls TED Embedding learning module initialized by
hirarchial clustering on tumor expression
profiles.
learn.embedding.withPhiTum
TED Embedding learning module with provided
tumor basis
norm.to.one Utility function to prepare the input.phi
run.Ted Bayesian deconvolution module
tinyi commented
Hi Qin,
Thank you for your question. *norm.to.one *is no longer needed, as it has
been internalized in the run.Ted function. Users can directly specify the
scRNA-seq input using the ref.dat argument of the run.Ted function without
the need to normalize it manually.
Please let me know if there are any questions.
Best,
Tinyi
…On Thu, Dec 30, 2021 at 2:25 AM Qin Lin ***@***.***> wrote:
Hi! I just install TED, but I couldn't find function, *norm.to.one*.
> TED::
TED::learn.embedding.withPhiTum TED::learn.embedding.Kcls TED::run.Ted TED::cleanup.genes TED::estimate_sf TED::get.signature.genes TED::convert.cell.fraction
>
> help(package="TED")
Information on package ‘TED’
Description:
Package: TED
Version: 1.1
Date: 2020-01-15
Title: BayesPrism: A Fully Bayesian Inference of Tumor
Microenvironment composition and gene expression.
Formerly called TED (Tumor microEnvironment
Deconvolution).
Author: Tinyi ***@***.***>, Charles G. Danko
***@***.***>
Maintainer: Tinyi ***@***.***>
Depends: R (>= 2.6)
Imports: DESeq2, parallel, MCMCpack, gplots, scran, BiocParallel
Description: TED is comprised of the deconvolution modules and the
embedding learning module. The deconvolution module
leverages cell type-specific expression profiles from
scRNA-seq and implements a fully Bayesian inference to
jointly estimate the posterior distribution of cell type
composition and cell type-specific gene expression from
bulk RNA-seq expression of tumor samples. The embedding
learning module uses Expectation-maximization (EM) to
approximate the tumor expression using a linear
combination of tumor pathways while conditional on the
inferred expression and fraction of non-tumor cells
estimated by the deconvolution module.
License: GPL-2 | GPL-3
biocViews: Sequencing, Analysis
LazyLoad: yes
RoxygenNote: 6.0.1
RemoteType: local
RemoteUrl: /home/user_li/linqin_tmp/SourceCode/ENIGMA/TED.zip
Built: R 4.0.3; ; 2021-12-26 11:38:32 UTC; unix
Index:
cleanup.genes Utility function to remove highly expressed
outlier genes that are sensitive to batch
effects from ref.dat
learn.embedding.Kcls TED Embedding learning module initialized by
hirarchial clustering on tumor expression
profiles.
learn.embedding.withPhiTum
TED Embedding learning module with provided
tumor basis
norm.to.one Utility function to prepare the input.phi
run.Ted Bayesian deconvolution module
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