ImagoXV/NanoASV

aarch64 - MK1C fail on minimal dataset

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Step 4/9 : Adapter trimming with Porechop
Step 5/9 : Subsampling
Step 6/9 : Reads alignements with bwa against SILVA_138.1
environment: line 1:   218 Segmentation fault      (core dumped) bwa mem ${DB}/SILVA_IDX "${FILE}" 2> /dev/null > "${FILE}.sam"
environment: line 1:   221 Segmentation fault      (core dumped) bwa mem ${DB}/SILVA_IDX "${FILE}" 2> /dev/null > "${FILE}.sam"
Step 7/9 : Skipped - no unknown sequence
Step 8/9 : Phylogeny with MAFFT and FastTree
Step 9/9 : Phylosequization with R and phyloseq
Data treatment is over.
NanoASV took 144 seconds to perform.

This indicates a memory related error.

If only the MK1C was running dozens of useless job in background.

I'll find a way

It worked with only one thread.

I highly doubt it can run with more than that.

EDIT : It does not run with 2 threads.

I hope increasing the number of sequences will not increase memory usage.

Maybe after changing bwa for bwa-mem2 IDK

Step 4/9 : Adapter trimming with Porechop
Step 5/9 : Subsampling
Step 6/9 : Reads alignements with bwa against SILVA_138.1
Step 7/9 : Skipped - no unknown sequence
Step 8/9 : Phylogeny with MAFFT and FastTree
Step 9/9 : Phylosequization with R and phyloseq
[1] "Exporting phyloseq object"
Data treatment is over.
NanoASV took 1076 seconds to perform.
Don't forget to cite NanoASV and its dependencies if it allows you to treat your data.