CpAverage or CrossingPoint

Question

CpAverage or CrossingPoint

lmanchon opened this issue 3 years ago · 6 comments

--Hi all,

I have a question about the output ixo files from the Lightcycler 480:
in these files there are the CpAverage and CrossingPoint values.
What values do I need to retrieve to use the pcr package?

Thanks

Answer 1 · 2021-07-16T11:43:17.000Z

Hi @lmanchon
I am not familiar with this particular machine. However, I think, it's the cp Average you should use. If you could provide an example file from the machine output, I might be able to confirm.
Cheers

Answer 2 · 2021-07-16T16:57:44.000Z

--Hi,

ok you can find an example in attachment here.
thanks.
TNFa.ixo.gz

Answer 3 · 2021-07-16T23:59:29.000Z

I can't open .ixo files on my machine. Is there a way to export the data into .txt files? If no, you could copy the relevant parts into a text file and share it.

Answer 4 · 2021-07-17T10:05:43.000Z

--
ixo file is just a text file.
You can open it with notepad+ on Windows machine or just with vim or vi on Linux system.

Answer 5 · 2021-07-17T11:28:21.000Z

Yes. I can. I am not sure however which of lines are relevant to the question.

Answer 6 · 2021-07-17T17:19:20.000Z

--
you can use grep to catch the lines with "CpAverage" or "CrossingPoint".
eaF1 is the gene control.