MahShaaban/pcr

pcr_wilcox function

lmanchon opened this issue · 4 comments

lmanchon commented

--Hi,

I get different pvalues when I use directly the wilcox.test function on my series of values.
Why does the pcr_wilcox function of the package return a different pvalue?

thank you --

MahShaaban commented

Would you please provide an example of the different values?

lmanchon commented

ok, sorry, i found my problem, you use a '.pcr_normalize' function in your package to normalize the values , i missed that.
But why to normalize the data when using a Wilcoxon test ?
The data must be normally distributed for a T-test and not for a Wilcoxon test, right ?

MahShaaban commented

.pcr_normalize normalize the gene of interest to the reference gene. This, I think, is quite different from the input values to the test functions being normally distributed or not.

lmanchon commented

ok.
not sure it makes much difference to normalize the values in the Wilcoxon test cases.