Handling different starting sample masses

Question

Handling different starting sample masses

JacobRPrice opened this issue 3 years ago · 3 comments

Is there a way to integrate sample mass (prior to DNA extraction) into our analysis, or scaling the results to account for different starting sample masses using the pcr package?

Answer 1 · 2022-02-04T07:15:16.000Z

The only way I can think of is to use pcr_test with the option test = 'lm'. You can include any arbitrary variable in a linear model and estimate/control for its effect.

Answer 2 · 2022-02-04T15:19:55.000Z

Hi @MahShaaban , thank you for the suggestion, I can give that a try.

At this point do you have any plans on developing or expanding the pcr package to include absolute abundance calculations and associated statistical tests? If so, the inclusion of sample mass information would be best implemented at that time.

Answer 3 · 2022-02-05T00:45:22.000Z

I am certainly open to the idea. If you like, you can kick start this feature by opening a new issue and providing some help on how it can be implemented.