Nanopore data filtering using fastp

[emilydolivo97]

Hello, I'm running the following command in my terminal:

fastp -i user/nanopore/inputs/ -o user/nanopore/outputs/ -A -G --qualified_quality_phred 10 --reads_to_process 5000 --thread 8

Although I'm specifying the number of reads to keep as 5000, after running my pipeline, I find that my fastq files have different numbers of reads such as 4965, 4778, and 4653, but never exactly 5000. Does anyone have an idea on how to fix this problem, please?