Russel88/CRISPRCasTyper

Execution against protein fasta and gff

Opened this issue · 2 comments

Hi,

First of all, I would like to thank you for developing this wonderful tool.

My question is about the input files of the tool.
Currently I have a genome sequence where gene prediction is a bit tricky. This genome cannot be predicted by prodigal and requires manual curation.

I would like to run CrisperCasTyper against this genome.
Is it possible to use gbk or protein fasta and gff as input?

Best,

Keigo

Hi Keigo,

It is unfortunately not possible to use other input than a nucleotide fasta. But it's definitely something I should implement, but it will be some time before I have the time to implement.

Best,
Jakob

I've made a pull request to allow to use GFF/Protein FASTA input instead of Prodigal, but it still needs the nucleotide FASTA for CRISPR arrays identification. For now it is only working with NCBI GFF, but making it work with prodigal should be easy