Skipping DATABASE

[airbender97]

Hello,

I have been adding a reference genome to fastq_screen.conf file but there are some problems.

I add this path to the .conf file:
## Western honey bee
DATABASE Apis_Mellifera /MGSvm_data/mpolat/apis_mellifera_ref/fasta

In the fasta folder I have my reference genome in fast format and five additional files produced by bwa index command.

"/MGSvm_data/mpolat/apis_mellifera_ref/fasta" this path contains the following files:

apis.fasta
apis.fasta.pac
apis.fasta.amb
apis.fasta.ann
apis.fasta.bwt
apis.fasta.sa

Whatever I do (I changed the file name into "apis", "apis.fasta", "fasta") but it gives the same error:

Using fastq_screen v0.15.1
Reading configuration from '/MGSvm_data/mpolat/fastq_screen/FastQ-Screen-0.15.2/fastq_screen.conf'
Using '/usr/bin/bwa' as BWA path
Skipping DATABASE 'Apis_Mellifera' since no BWA index was found at '/MGSvm_data/mpolat/apis_mellifera_ref/fasta'
Using 8 threads for searches
No reference genomes were configured, please adjust configuration.

I need help 👯

[airbender97]

Okay I found the problem... Sometimes my brains does not get enough oxygen. In the path, I have to specify the fasta file as well.

/MGSvm_data/mpolat/apis_mellifera_ref/fasta/apis.fasta