The importSTARsolo function does not load the Summary.csv file if using the GeneFull folder instead of the Gene folder
apeleraux opened this issue · 1 comments
When aligning with STARsolo, you can use the --soloFeatures GeneFull option to include introns in the alignment. The alignment results are found in a folder called GeneFull (rather than Gene) in the Solo.out folder, but the organization of the STARsolo output files is otherwise exactly the same.
When importing results from the GeneFull folders, importSTARsolo function does not find the Summary.csv files, therefore a warning is shown for each sample, saying "Metrics summary file (Summary.csv) not found for sample: xxx".
Example code:
sce <- importSTARsolo(
STARsoloDirs = path_list, # paths to solo.out folders
STARsoloOuts="GeneFull/filtered", # instead of "Gene/filtered"
matrixFileNames = "matrix.mtx",
featuresFileNames = "features.tsv",
barcodesFileNames = "barcodes.tsv",
samples = sample_list)
This has been fixed in v2.8.1.