STAR/HiSAT2 output coordinate sorted BAM
Closed this issue · 2 comments
lindaxiang commented
@akahles Can we sort STAR/HiSAT2 output BAMs by coordinate? Otherwise the index file can not be built.
akahles commented
We usually do this as a downstream process after alignment. The STAR sorting (at least in the past) was not very efficient and using samtools view knew that the output was compatible with downstream tools.
lindaxiang commented
This has been addressed.