Combination of qPCR and BLAST db fails

[elischberg]

  File "/usr/local/tools/_conda/envs/mulled-v1-88ec11123572a51b245ba097dc0b273940132dc7e25597f386c2d705a3f21960/bin/varvamp", line 10, in <module>
    sys.exit(main())
  File "/usr/local/tools/_conda/envs/mulled-v1-88ec11123572a51b245ba097dc0b273940132dc7e25597f386c2d705a3f21960/lib/python3.10/site-packages/varvamp/command.py", line 557, in main
    probe_regions, final_schemes = qpcr_workflow(
  File "/usr/local/tools/_conda/envs/mulled-v1-88ec11123572a51b245ba097dc0b273940132dc7e25597f386c2d705a3f21960/lib/python3.10/site-packages/varvamp/command.py", line 455, in qpcr_workflow
    amplicons, off_target_amplicons = blast.primer_blast(
  File "/usr/local/tools/_conda/envs/mulled-v1-88ec11123572a51b245ba097dc0b273940132dc7e25597f386c2d705a3f21960/lib/python3.10/site-packages/varvamp/scripts/blast.py", line 240, in primer_blast
    off_target_amplicons, amplicons = predict_non_specific_amplicons(
  File "/usr/local/tools/_conda/envs/mulled-v1-88ec11123572a51b245ba097dc0b273940132dc7e25597f386c2d705a3f21960/lib/python3.10/site-packages/varvamp/scripts/blast.py", line 206, in predict_non_specific_amplicons
    amplicons[off_target]["penalty"][0] = amplicons[off_target]["penalty"][0] + config.BLAST_PENALTY
TypeError: 'float' object is not subscriptable```

[jonas-fuchs]

Ok yeah that seems to be a pretty stupid bug, should be an easy fix I hope. I will link an pr tomorrow. Can you do a test afterwards?

[elischberg]

It's working. (: