Differential expression error
jfad1286 opened this issue · 4 comments
Hi. I was wondering if you could help me. I have some RNA Seq data that I wanted to analyse and so far I've managed to follow the guide all the way to the differential expression analysis. I get an error and I'm not sure how to solve it. I attach the log from the analysis error.
I have data from 6 animals, 3 wild type (WT) and 3 knock-outs (KO). I created the groups for both with three animals in each. Then I created another group with both WT and KO groups inside, this allowed me to select the "sample group" (their genotype in this case) as the contrast variables. I left all other options in their default settings, although because of the error I also tried changing different options, but I haven't been able to make it work.
Could the way I created the analysis groups be the issue, how can I solve it if it is?
Thanks for your help!
Juan
Dear Juan,
Thank you for the report. I need to run some tests to determine the exact source of your problem.
I will let you know if I need more details about the analysis you are trying to run in order to determine the issue.
I traced the problem to a corrupted R package. As a result, I've updated and rebuilt the RNAdetector docker container.
Next time, when you start the app with an internet connection, you should be notified of a new update (the download is automatic).
Please, let me know if this solves your issue.
Dear Juan,
I wonder whether you have solved the problem?
I experience exactly the same.
Thanks,
Yulia